liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Gut commensal bacteria and regional Wnt gene expression in the proximal versus distal colon
Epithelial Pathobiology and Mucosal Inflammation Research Unit, Emory University, Atlanta, Georgia; Department of General and Visceral Surgery, University of Muenster, Muenster, Germany.
Epithelial Pathobiology and Mucosal Inflammation Research Unit, Emory University, Atlanta, Georgia; Division of Molecular Embryology, German Cancer Research Center, Heidelberg, Germany.
Epithelial Pathobiology and Mucosal Inflammation Research Unit, Emory University, Atlanta, Georgia.
Department of Pharmacology, University of North Carolina,Chapel Hill, Chapel Hill, North Carolina.
Show others and affiliations
2014 (English)In: American Journal of Pathology, ISSN 0002-9440, E-ISSN 1525-2191, Am J Pathol, Vol. 184, no 3, 592-9 p.Article in journal (Refereed) Published
Abstract [en]

Regional expression of Wingless/Int (Wnt) genes plays a central role in regulating intestinal development and homeostasis. However, our knowledge of such regional Wnt proteins in the colon remains limited. To understand further the effect of Wnt signaling components in controlling intestinal epithelial homeostasis, we investigated whether the physiological heterogeneity of the proximal and distal colon can be explained by differential Wnt signaling. With the use of a Wnt signaling-specific PCR array, expression of 84 Wnt-mediated signal transduction genes was analyzed, and a differential signature of Wnt-related genes in the proximal versus distal murine colon was identified. Several Wnt agonists (Wnt5a, Wnt8b, and Wnt11), the Wnt receptor frizzled family receptor 3, and the Wnt inhibitory factor 1 were differentially expressed along the colon length. These Wnt signatures were associated with differential epithelial cell proliferation and migration in the proximal versus distal colon. Furthermore, reduced Wnt/beta-catenin activity and decreased Wnt5a and Wnt11 expression were observed in mice lacking commensal bacteria, an effect that was reversed by conventionalization of germ-free mice. Interestingly, myeloid differentiation primary response gene 88 knockout mice showed decreased Wnt5a levels, indicating a role for Toll-like receptor signaling in regulating Wnt5a expression. Our results suggest that the morphological and physiological heterogeneity within the colon is in part facilitated by the differential expression of Wnt signaling components and influenced by colonization with bacteria.

Place, publisher, year, edition, pages
Elsevier, 2014. Vol. 184, no 3, 592-9 p.
Keyword [en]
Animals, Bacteria/*metabolism, Cell Proliferation, Colon/anatomy & histology/metabolism/*microbiology, Gene Expression Regulation, Bacterial, Mice, Mice, Inbred C57BL, Mice, Knockout, Microbiota, *Signal Transduction, Specific Pathogen-Free Organisms, Wnt Proteins/genetics/*metabolism, Wnt-5a Protein
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-141667DOI: 10.1016/j.ajpath.2013.11.029ISI: 000332055500003PubMedID: 24418259Scopus ID: 2-s2.0-84894111787ISBN: 1525-2191 (Electronic) 0002-9440 (Linking) OAI: oai:DiVA.org:liu-141667DiVA: diva2:1149704
Note

Neumann, Philipp-Alexander Koch, Stefan Hilgarth, Roland S Perez-Chanona, Ernesto Denning, Patricia Jobin, Christian Nusrat, Asma eng R01 DK073338/DK/NIDDK NIH HHS/ R01HD059122/HD/NICHD NIH HHS/ R01 HD059122/HD/NICHD NIH HHS/ R01 DK055679/DK/NIDDK NIH HHS/ DK059888/DK/NIDDK NIH HHS/ R01 DK059888/DK/NIDDK NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2014/01/15 06:00 Am J Pathol. 2014 Mar;184(3):592-9. doi: 10.1016/j.ajpath.2013.11.029. Epub 2014 Jan 11.

Available from: 2017-10-16 Created: 2017-10-16 Last updated: 2017-10-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Authority records BETA

Koch, Stefan

Search in DiVA

By author/editor
Koch, Stefan
In the same journal
American Journal of Pathology
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
isbn
urn-nbn

Altmetric score

doi
pubmed
isbn
urn-nbn
Total: 54 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf