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Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates.
Istanbul Kemerburgaz University, School of Medicine, Istanbul 34217, Turkey.
Yeditepe University, School of Medicine, Department of Biophysics, Istanbul Turkey.
SOMAprobes S.L., Science and Technology Park of Gipuzkoa, San Sebastian 20009, Spain.
SOMAprobes S.L., Science and Technology Park of Gipuzkoa, San Sebastian 20009, Spain.
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 86, 27-32 p.Article in journal (Refereed) Published
Abstract [en]

A fast, specific and sensitive homogeneous assay for Staphylococcus aureus detection was developed by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucleotide. As biosensor format, an effective system, Nanokeepers as previously reported, were used for triggered release of confined fluorophores, and hence specific detection of S. aureus on nuclease activity was obtained. The interference from blood components for fluorescent quantification was eliminated by a pre-purification by aptamer-functionalized silica magnetic nanoparticles. The reported assay system was exclusively formed by nucleic acid oligos and magnetic or mesoporous silica nanoparticles, that can be used on blood samples in a stepwise manner. The assay was successfully used as a sensing platform for the specific detection of S. aureus cells as low as 682 CFU in whole blood.

Place, publisher, year, edition, pages
2016. Vol. 86, 27-32 p.
Keyword [en]
Aptamers, Magnetic separation, Micrococcal nuclease, Nanoparticles, Staphylococcus aureus
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:liu:diva-142936DOI: 10.1016/j.bios.2016.06.023PubMedID: 27318566OAI: oai:DiVA.org:liu-142936DiVA: diva2:1156444
Available from: 2017-11-13 Created: 2017-11-13 Last updated: 2017-12-04

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Hernandez, Frank J
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