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Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamers
Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain.
Photonics and Optoelectronics Group, Physics Department and Center for Nanoscience CeNS, Ludwig-Maximilians-Universität München, Munich, Germany.
Bioengineering and Bioelectrochemistry Group, Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain.
Bioengineering and Bioelectrochemistry Group, Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain.
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2009 (English)In: Journal of Biophotonics, ISSN 1864-063X, E-ISSN 1864-0648, Vol. 2, no 4, 227-231 p.Article in journal (Refereed) Published
Abstract [en]

Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta-nano-sensor is in the range of 20 nM. Due to its nanoscopic size, this single nanoparticle based apta-sensor may be used in nanoscopic volumes such as in array type assays or even inside cells.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2009. Vol. 2, no 4, 227-231 p.
Keyword [en]
apatamer, gold nanoparticles, biosensing
National Category
Biophysics
Identifiers
URN: urn:nbn:se:liu:diva-142958DOI: 10.1002/jbio.200910006ISI: 000265856000009PubMedID: 19367590Scopus ID: 2-s2.0-66949168506OAI: oai:DiVA.org:liu-142958DiVA: diva2:1156474
Available from: 2017-11-13 Created: 2017-11-13 Last updated: 2017-11-29Bibliographically approved

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Hernandez, Frank Jeyson
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