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Stallion spermatozoa surviving freezing and thawing experience membrane depolarization and increased intracellular Na+
University of Leon, Spain.
University of Leon, Spain.
University of Extremadura, Spain.
University of Extremadura, Spain.
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2017 (English)In: Andrology, ISSN 2047-2919, E-ISSN 2047-2927, Vol. 5, no 6, p. 1174-1182Article in journal (Refereed) Published
Abstract [en]

In order to gain insight of the modifications that freezing and thawing cause to the surviving population of spermatozoa, changes in the potential of the plasma membrane (Em) and intracellular Na+ content of stallion spermatozoa were investigated using flow cytometry. Moreover, caspase 3 activity was also investigated and the functionality of the Na+-K+ ATPase pump was investigated before and after freezing and thawing. Cryopreservation caused a significant (pamp;lt;0.001) increase in the subpopulation of spermatozoa with depolarized sperm membranes, concomitantly with an increase (pamp;lt;0.05) in intracellular Na+. These changes occurred in relation to activation of caspase 3 (pamp;lt;0.001). Cryopreservation reduced the activity of the Na-K+ pump and inhibition of the Na+-K+ ATPase pump with ouabain-induced caspase 3 activation. It is concluded that inactivation of Na+-K+ ATPase occurs during cryopreservation, an inhibition that could play a role explaining the accelerated senescence of the surviving population of spermatozoa.

Place, publisher, year, edition, pages
WILEY , 2017. Vol. 5, no 6, p. 1174-1182
Keywords [en]
ATP; cryopreservation; flow cytometry; horse; Na+-K+; ATPase; sperm
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:liu:diva-143075DOI: 10.1111/andr.12419ISI: 000414411400014PubMedID: 28973824OAI: oai:DiVA.org:liu-143075DiVA, id: diva2:1159446
Note

Funding Agencies|Ministerio de Economia y Competitividad-FEDER, Madrid, Spain [AGL2013-43211-R]; Junta de Extremadura-FEDER [GR 15029, IB16030]; Swedish Research Councils VR [521-2011-6353]; Formas [221-2011-512]; Ministerio de Educacion, Cultura y Deporte, Madrid Spain [FPU13/03991]; Ministerio de Economia y Competitividad Juan de la Cierva [IJCI-2014-21671]

Available from: 2017-11-22 Created: 2017-11-22 Last updated: 2017-12-11

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Rodriguez-Martinez, Heriberto
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Division of Children's and Women's healthFaculty of Medicine and Health Sciences
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