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Staphylococcus aureus Nuc2 is a functional, surface-attached extracellular nuclease
Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of of Iowa, Iowa City, IA, United States.
Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of of Iowa, Iowa City, IA, United States.
Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of of Iowa, Iowa City, IA, United States.
Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of of Iowa, Iowa City, IA, United States.
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2014 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 9, no 4, e95574Article in journal (Refereed) Published
Abstract [en]

Staphylococcus aureus is a prominent bacterial pathogen that causes a diverse range of acute and chronic infections. Recently, it has been demonstrated that the secreted nuclease (Nuc) enzyme is a virulence factor in multiple models of infection, and in vivo expression of nuc has facilitated the development of an infection imaging approach based on Nuc-activatable probes. Interestingly, S. aureus strains encode a second nuclease (Nuc2) that has received limited attention. With the growing interest in bacterial nucleases, we sought to characterize Nuc2 in more detail through localization, expression, and biochemical studies. Fluorescence microscopy and alkaline phosphatase localization approaches using Nuc2-GFP and Nuc2-PhoA fusions, respectively, demonstrated that Nuc2 is membrane bound with the C-terminus facing the extracellular environment, indicating it is a signal-anchored Type II membrane protein. Nuc2 enzyme activity was detectable on the S. aureus cell surface using a fluorescence resonance energy transfer (FRET) assay, and in time courses, both nuc2 transcription and enzyme activity peaked in early logarithmic growth and declined in stationary phase. Using a mouse model of S. aureus pyomyositis, Nuc2 activity was detected with activatable probes in vivo in nuc mutant strains, demonstrating that Nuc2 is produced during infections. To assess Nuc2 biochemical properties, the protein was purified and found to cleave both single- and double-stranded DNA, and it exhibited thermostability and calcium dependence, paralleling the properties of Nuc. Purified Nuc2 prevented biofilm formation in vitro and modestly decreased biomass in dispersal experiments. Altogether, our findings confirm that S. aureus encodes a second, surface-attached and functional DNase that is expressed during infections and displays similar biochemical properties to the secreted Nuc enzyme. © 2014 Kiedrowski et al.

Place, publisher, year, edition, pages
San Francisco, United States: Public Library of Science , 2014. Vol. 9, no 4, e95574
National Category
Microbiology in the medical area
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URN: urn:nbn:se:liu:diva-143331DOI: 10.1371/journal.pone.0095574ISI: 000335227400078PubMedID: 24752186Scopus ID: 2-s2.0-84899842878OAI: oai:DiVA.org:liu-143331DiVA: diva2:1162487
Note

Funding Agencies|AI083211, NIH, National Institutes of Health; AI101391, NIH, National Institutes of Health

Available from: 2017-12-04 Created: 2017-12-04 Last updated: 2017-12-12Bibliographically approved

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Hernandez, Frank J

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