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Molecular determinants for the recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin.
Division of Structural Biology, Biozentrum, University of Basel, Basel, Switzerland.
Institut für Anästhesiologie und Operative Intensivmedizin, Universitätsklinikum Mannheim, Mannheim, Germany.
Institut für Anästhesiologie und Operative Intensivmedizin, Universitätsklinikum Mannheim, Mannheim, Germany.
Chemical Biophysics, Biozentrum, University of Basel, Basel, Switzerland.
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2007 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 21, no 7, p. 1383-1392Article in journal (Refereed) Published
Abstract [en]

Titin forms an intrasarcomeric filament system in vertebrate striated muscle, which has elastic and signaling properties and is thereby central to mechanotransduction. Near its C-terminus and directly preceding a kinase domain, titin contains a conserved pattern of Ig and FnIII modules (Ig(A168)-Ig(A169)-FnIII(A170), hereby A168-A170) that recruits the E3 ubiquitin-ligase MuRF-1 to the filament. This interaction is thought to regulate myofibril turnover and the trophic state of muscle. We have elucidated the crystal structure of A168-A170, characterized MuRF-1 variants by circular dichroism (CD) and SEC-MALS, and studied the interaction of both components by isothermal calorimetry, SPOTS blots, and pull-down assays. This has led to the identification of the molecular determinants of the binding. A168-A170 shows an extended, rigid architecture, which is characterized by a shallow surface groove that spans its full length and a distinct loop protrusion in its middle point. In MuRF-1, a C-terminal helical domain is sufficient to bind A168-A170 with high affinity. This helical region predictably docks into the surface groove of A168-A170. Furthermore, pull-down assays demonstrate that the loop protrusion in A168-A170 is a key mediator of MuRF-1 recognition. Our findings indicate that this region of titin could serve as a target to attempt therapeutic inhibition of MuRF-1-mediated muscle turnover, where binding of small molecules to its distinctive structural features could block MuRF-1 access.

Place, publisher, year, edition, pages
2007. Vol. 21, no 7, p. 1383-1392
Keywords [en]
elastic filament titin, muscle atrophy, X-ray crystallography, binding studies
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-143656DOI: 10.1096/fj.06-7644comISI: 000246117000014PubMedID: 17215480Scopus ID: 2-s2.0-34247624040OAI: oai:DiVA.org:liu-143656DiVA, id: diva2:1165087
Available from: 2017-12-12 Created: 2017-12-12 Last updated: 2017-12-19Bibliographically approved

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von Castelmur, Eleonore

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