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Comparisons between commercial salivary testosterone enzyme-linked immunosorbent assay kits
Örebro University, Sweden.
Uppsala University, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry. Örebro University, Sweden.
2017 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 77, no 8, p. 582-586Article in journal (Refereed) Published
Abstract [en]

Introduction: Measuring testosterone concentrations is of interest both in clinical situations and for research, the latter expanding rapidly during recent years. An increased demand for convenient methods has prompted a number of companies to develop enzyme-linked immunosorbent assay (ELISA) kits to measure testosterone concentrations in saliva. However, the inter-comparability of kits from different manufacturers have yet to be determined. Aim of study: The aim of this study was to compare commercially available ELISA kits from four different manufacturers (Salimetrics, IBL, DRG and Demeditec). Methods: Saliva was collected from 50 participants (25 men and 25 women). Each sample was analysed by the four ELISA kits. Results: The correlations between the ELISA kits from Demeditec, DRG and Salimetrics were moderate to high with r-values amp;gt;.77; however, proportional errors between the methods calls for caution. The ELISA kit from IBL malfunctioned and no results from this kit was obtained. Conclusions: Results from studies using the ELISA kits from Demeditec, DRG and Salimetrics are generally comparable; however, translation using the formulae presented in the current study could increase the accuracy of these comparisons.

Place, publisher, year, edition, pages
TAYLOR & FRANCIS LTD , 2017. Vol. 77, no 8, p. 582-586
Keywords [en]
Androgens; immunoassay; methods; saliva; testosterone
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:liu:diva-143753DOI: 10.1080/00365513.2017.1339231ISI: 000416756100004PubMedID: 28644096OAI: oai:DiVA.org:liu-143753DiVA, id: diva2:1166630
Note

Funding Agencies|Linkoping University; Ostergotland County; Orebro University; Region Orebro lan; Forskningskommitten; Swedish Research Council [SRC 2015-4870]

Available from: 2017-12-15 Created: 2017-12-15 Last updated: 2018-05-03

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Theodorsson, ElvarStröm, Jakob
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Division of Microbiology and Molecular MedicineFaculty of Medicine and Health SciencesDepartment of Clinical Chemistry
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