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An inter-laboratory comparison study on transfer, persistence and recovery of DNA from cable ties
Netherlands Forens Institute, Netherlands.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, Faculty of Science & Engineering. Swedish National Forens Centre, SE-58194 Linkoping, Sweden.
Netherlands Forens Institute, Netherlands.
Forens Science Ireland, Ireland.
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2017 (English)In: Forensic Science International: Genetics, ISSN 1872-4973, E-ISSN 1878-0326, Vol. 31, p. 95-104Article in journal (Refereed) Published
Abstract [en]

To address questions on the activity that led to the deposition of biological traces in a particular case, general information on the probabilities of transfer, persistence and recovery of cellular material in relevant scenarios is necessary. These figures may be derived from experimental data described in forensic literature when conditions relevant to the case were included. The experimental methodology regarding sampling, DNA extraction, DNA typing and profile interpretation that were used to generate these published data may differ from those applied in the case and thus the applicability of the literature data may be questioned. To assess the level of variability that different laboratories obtain when similar exhibits are analysed, we performed an inter-laboratory study between four partner laboratories. Five sets of 20 cable ties bound by different volunteers were distributed to the participating laboratories and sampled and processed according to the in-house protocols. Differences were found for the amount of retrieved DNA, as well as for the reportability and composition of the DNA profiles. These differences also resulted in different probabilities of transfer, persistence and recovery for each laboratory. Nevertheless, when applied to a case example, these differences resulted in similar assignments of weight of evidence given activity-level propositions.

Place, publisher, year, edition, pages
ELSEVIER IRELAND LTD , 2017. Vol. 31, p. 95-104
Keywords [en]
Forensic science; DNA transfer; DNA persistence; Tie-wrap; Cable tie; Criminalistics; Activity-level reporting
National Category
Medical Genetics
Identifiers
URN: urn:nbn:se:liu:diva-143998DOI: 10.1016/j.fsigen.2017.08.015ISI: 000417098900029PubMedID: 28858675OAI: oai:DiVA.org:liu-143998DiVA, id: diva2:1170210
Note

Funding Agencies|European Commission through European Network of Forensic Science Institutes (ENFSI) [MP2013 T5]; Prevention of and Fight against Crime Programme of the European Union [HOME/2013/ISEC/MO/ENFSI/4000005962]

Available from: 2018-01-02 Created: 2018-01-02 Last updated: 2018-01-13

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
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  • Other style
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  • de-DE
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  • nn-NB
  • sv-SE
  • Other locale
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Output format
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