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RapidHIT for the purpose of stain analyses – An interrupted implementation
Swedish National Forensic Centre, Linköping, Sweden.
Swedish National Forensic Centre, Linköping, Sweden.
Swedish National Forensic Centre, Linköping, Sweden.
Swedish National Forensic Centre, Linköping, Sweden / Applied Microbiology, Department of Chemistry, Lund University, Lund, Sweden.
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2017 (English)In: Forensic Science International: Genetics Supplement Series, ISSN 1875-1768, E-ISSN 1875-175X, Vol. 6, no Supplement C, p. e589-e590Article in journal (Refereed) Published
Abstract [en]

Rapid DNA instruments have in recent years been developed, enabling analysis of forensic samples with a minimum of human intervention. Initially intended for fast handling of reference samples, such as samples from suspects in booking suites, attention shifted to include crime scene samples. The aim of this study was to determine whether or not the RapidHIT System (IntegenX) is fit for crime scene samples. The first runs gave very poor results, which was found to be due to an incorrect firmware setting leading to no or just minute amounts of amplicons being injected for electrophoresis. After solving this problem, 28 full runs (seven samples each) applying NGM SElect Express were performed comprising various amounts of blood on cotton swabs. Six of the runs failed completely, four due to cartridge leakage and in two runs the PCR mix was not injected. For 155 samples with 1–5ÎŒL blood (volumes for which complete DNA profiles are expected), 119 samples (77%) gave complete DNA profiles. Among the most serious failures were incorrect allele calling and leakage of DNA extract or PCR product. Other general issues were failure to export results, anode motor breakdown and broken capillary array. Due to the encountered problems with software, hardware and cartridges, together with the low success rate, it was decided not to continue towards implementation of the RapidHIT System in casework.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 6, no Supplement C, p. e589-e590
Keywords [en]
Automation, Forensic DNA analyses, Rapid DNA, RapidHIT System, STR
National Category
Analytical Chemistry Biomedical Laboratory Science/Technology Forensic Science
Identifiers
URN: urn:nbn:se:liu:diva-144104DOI: 10.1016/j.fsigss.2017.10.002OAI: oai:DiVA.org:liu-144104DiVA, id: diva2:1170919
Available from: 2018-01-05 Created: 2018-01-05 Last updated: 2018-01-05

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Ansell, Ricky

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