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5-Hydroxymethylcytosine Profiling in Human DNA
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road, Edinburgh, UK.
Linköping University, Department of Clinical and Experimental Medicine, Division of Children's and Women's health. Linköping University, Faculty of Medicine and Health Sciences. (The Centre for Individualized Medication)
MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, The University of Edinburgh, Crewe Road, Edinburgh, UK.
2017 (English)In: Population Epigenetics: Methods and Protocols / [ed] Paul Haggarty; Kristina Harrison, Humana Press, 2017, Vol. 1589, p. 89-98Chapter in book (Refereed)
Abstract [en]

Since its "re-discovery" in 2009, there has been significant interest in defining the genome-wide distribution of DNA marked by 5-hydroxymethylation at cytosine bases (5hmC). In recent years, technological advances have resulted in a multitude of unique strategies to map 5hmC across the human genome. Here we discuss the wide range of approaches available to map this modification and describe in detail the affinity based methods which result in the enrichment of 5hmC marked DNA for downstream analysis.

Place, publisher, year, edition, pages
Humana Press, 2017. Vol. 1589, p. 89-98
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1589
Keyword [en]
5-hydroxymethylcytosine; 5hmC; 5mC; DNA immunoprecipitation and enrichment; Epigenetics; HmeDIP
National Category
Genetics
Identifiers
URN: urn:nbn:se:liu:diva-145072DOI: 10.1007/7651_2015_268PubMedID: 26126447ISBN: 978-1-4939-6901-2 (print)ISBN: 978-1-4939-6903-6 (electronic)OAI: oai:DiVA.org:liu-145072DiVA, id: diva2:1181225
Available from: 2018-02-08 Created: 2018-02-08 Last updated: 2018-02-09

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Nestor, Colm

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
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  • de-DE
  • en-GB
  • en-US
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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