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Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Department of Clinical Medicine, Örebro University, Örebro, Sweden.ORCID iD: 0000-0002-1920-3962
2017 (English)In: Lysosomes: Methods and Protocols / [ed] Karin Öllinger; Hanna Appelqvist, Humana Press, 2017, Vol. 1594, p. 191-203Chapter in book (Refereed)
Abstract [en]

Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.

Place, publisher, year, edition, pages
Humana Press, 2017. Vol. 1594, p. 191-203
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1594
Keyword [en]
Flow cytometry; Granule; Lysosomal exocytosis; Lysosomal-associated membrane protein-1 (LAMP-1); Lysosome; Platelet; Platelet activation; Platelet secretion
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:liu:diva-145058DOI: 10.1007/978-1-4939-6934-0_12PubMedID: 28456984ISBN: 978-1-4939-6932-6 (print)ISBN: 978-1-4939-6934-0 (electronic)OAI: oai:DiVA.org:liu-145058DiVA: diva2:1181722
Available from: 2018-02-09 Created: 2018-02-09 Last updated: 2018-02-09

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CiteExportLink to record
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