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Simple storage (CO2-free) of porcine morulae for up to three days maintains the in vitro viability and developmental competence
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
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2018 (English)In: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 108, p. 229-238Article in journal (Refereed) Published
Abstract [en]

The advancement of porcine embryo transfer (ET) technology is constrained by regulatory hurdles (liquid nitrogen transportation) or, more importantly, the technical obstacles of using vitrified embryos in combination with nonsurgical deep uterine ET technology. Maintaining embryos in culture during transport and prior ET collides with the need of CO2 gassing and the best choice of culture medium. In this work, we describe storage conditions for short-term embryo CO2-free storage that allowed for a majority of in vivo-derived porcine morulae to survive after 3 days of storage in a liquid state, and to develop to the blastocyst stage unhatched, a sanitary prerequisite for ET. The storage conditions included NCSU-23 medium supplemented with bovine serum albumin, where bicarbonate was partially replaced by HEPES to avoid the need for CO2 gassing, and a temperature of 37 degrees C. These conditions were able to maintain the functionality of the stored embryos (hatching capacity after exposure to conventional culture conditions) and their developmental competence after ET (normal fetuses by day 38 of pregnancy). Use of this strategy of CO2-free storage should allow the shipment of fresh embryos worldwide in the absence of liquid nitrogen. (C) 2017 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC , 2018. Vol. 108, p. 229-238
Keyword [en]
CO2-free embryo storage; Morulae; Blastocyst; Embryo transfer; Pig
National Category
Obstetrics, Gynecology and Reproductive Medicine
Identifiers
URN: urn:nbn:se:liu:diva-145440DOI: 10.1016/j.theriogenology.2017.12.001ISI: 000424732900029PubMedID: 29253666OAI: oai:DiVA.org:liu-145440DiVA, id: diva2:1194600
Note

Funding Agencies|Ministry of Economy and Competitiveness (Madrid, Spain)/the European Regional Development Fund [RTC-2016-5448-2, AGL2015-69735-R]; Seneca Foundation, Murcia, Spain [19892/GERM/15]; Swedish Research Council Formas, Stockholm, Sweden [2017-00946]

Available from: 2018-04-03 Created: 2018-04-03 Last updated: 2018-04-03

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Rodriguez-Martinez, Heriberto
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Division of Children's and Women's healthFaculty of Medicine and Health Sciences
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