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Labeling nanoparticles: Dye leakage and altered cellular uptake
Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway.
Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway; Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway.
Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway.
Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway; SINTEF Materials and Chemistry, Trondheim, Norway.
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2017 (English)In: Cytometry Part A, ISSN 1552-4922, E-ISSN 1552-4930, Vol. 91, no 8, p. 760-766Article in journal (Refereed) Published
Abstract [en]

In vitro and in vivo behavior of nanoparticles (NPs) is often studied by tracing the NPs with fluorescent dyes. This requires stable incorporation of dyes within the NPs, as dye leakage may give a wrong interpretation of NP biodistribution, cellular uptake, and intracellular distribution. Furthermore, NP labeling with trace amounts of dye should not alter NP properties such as interactions with cells or tissues. To allow for versatile NP studies with a variety of fluorescence-based assays, labeling of NPs with different dyes is desirable. Hence, when new dyes are introduced, simple and fast screening methods to assess labeling stability and NP-cell interactions are needed. For this purpose, we have used a previously described generic flow cytometry assay; incubation of cells with NPs at 4 and 37C. Cell-NP interaction is confirmed by cellular fluorescence after 37C incubation, and NP-dye retention is confirmed when no cellular fluorescence is detected at 4C. Three different NP-platforms labeled with six different dyes were screened, and a great variability in dye retention was observed. Surprisingly, incorporation of trace amounts of certain dyes was found to reduce or even inhibit NP uptake. This work highlights the importance of thoroughly evaluating every dye-NP combination before pursuing NP-based applications. © 2016 International Society for Advancement of Cytometry.

Place, publisher, year, edition, pages
John Wiley & Sons, 2017. Vol. 91, no 8, p. 760-766
Keyword [en]
cellular uptake; flow cytometry; leakage; liposomes; nanoemulsions; polymeric nanoparticles
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:liu:diva-146318DOI: 10.1002/cyto.a.22853ISI: 000408333700004PubMedID: 27077940Scopus ID: 2-s2.0-84963800614OAI: oai:DiVA.org:liu-146318DiVA, id: diva2:1195944
Available from: 2018-04-07 Created: 2018-04-07 Last updated: 2018-04-19Bibliographically approved

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Bäck, MarcusNilsson, K. Peter R.

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