liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Proteomic Analyses of the Unexplored Sea Anemone Bunodactis verrucosa
Univ Porto, Portugal.
Univ Porto, Portugal.
Hanover Med Sch MHH, Germany.
Linköping University, Department of Clinical and Experimental Medicine, Divison of Neurobiology. Linköping University, Faculty of Medicine and Health Sciences.
Show others and affiliations
2018 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 16, no 2, article id 42Article in journal (Refereed) Published
Abstract [en]

Cnidarian toxic products, particularly peptide toxins, constitute a promising target for biomedicine research. Indeed, cnidarians are considered as the largest phylum of generally toxic animals. However, research on peptides and toxins of sea anemones is still limited. Moreover, most of the toxins from sea anemones have been discovered by classical purification approaches. Recently, high-throughput methodologies have been used for this purpose but in other Phyla. Hence, the present work was focused on the proteomic analyses of whole-body extract from the unexplored sea anemone Bunodactis verrucosa. The proteomic analyses applied were based on two methods: two-dimensional gel electrophoresis combined with MALDI-TOF/TOF and shotgun proteomic approach. In total, 413 proteins were identified, but only eight proteins were identified from gel-based analyses. Such proteins are mainly involved in basal metabolism and biosynthesis of antibiotics as the most relevant pathways. In addition, some putative toxins including metalloproteinases and neurotoxins were also identified. These findings reinforce the significance of the production of antimicrobial compounds and toxins by sea anemones, which play a significant role in defense and feeding. In general, the present study provides the first proteome map of the sea anemone B. verrucosa stablishing a reference for future studies in the discovery of new compounds.

Place, publisher, year, edition, pages
MDPI , 2018. Vol. 16, no 2, article id 42
Keywords [en]
cnidarian; sea anemone; proteins; toxins; two-dimensional gel electrophoresis; MALDI-TOF; TOF; shotgun proteomic
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-147249DOI: 10.3390/md16020042ISI: 000427528800004PubMedID: 29364843OAI: oai:DiVA.org:liu-147249DiVA, id: diva2:1197202
Note

Funding Agencies|Portuguese Foundation for Science and Technology (FCTFundacAo para a Ciencia e a Tecnologia, Portugal) [SFRH/BD/80592/2011]; FCT [SFRH/BPD/92978/2013, SFRH/BPD/103683/2014]; Alexander von Humboldt postdoctoral fellowship (Collaborative Research Centre -German Research Foundation) [3.2-KUB/1153731, 1279]; FCT and the European Regional Development Fund (ERDF) [UID/Multi/04423/2013]; European Structural and Investment Funds (ESIF) through the Competitiveness and Internationalization Operational ProgramCOMPETE 2020; National Funds through the FCT [PTDC/AAG-GLO/6887/2014, (POCI-01-0124-FEDER-016845)]; Structured Programs of R&D&I INNOVMARInnovation and Sustainability in the Management and Exploitation of Marine Resources [NORTE-01-0145-FEDER-000035]; CORAL NORTE [NORTE-01-0145-FEDER-000036]; Northern Regional Operational Program (NORTE) through the ERDF

Available from: 2018-04-12 Created: 2018-04-12 Last updated: 2018-04-12

Open Access in DiVA

fulltext(2654 kB)55 downloads
File information
File name FULLTEXT01.pdfFile size 2654 kBChecksum SHA-512
075d77082f28b6829a74c14fea33f2087753b4fcbdf2dbd6a8a319f2160ea20ca6bed4ec5af0fe1b8713bf6557dce08d95f1235aaee37592524d67bac734bc9b
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Turkina, Maria
By organisation
Divison of NeurobiologyFaculty of Medicine and Health Sciences
In the same journal
Marine Drugs
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 55 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 174 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf