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Molecular analysis of the fungal microbiome associated with the olive fruit fly Bactrocera oleae
University of Reggio Calabria, Italy; Universita of Palermo, Palermo, Italy.ORCID iD: 0000-0002-0811-1229
University of Reggio Calabria, Italy.
University of Reggio Calabria, Italy.
University of Reggio Calabria, Italy.
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2015 (English)In: Fungal ecology, ISSN 1754-5048, E-ISSN 1878-0083, Vol. 18, p. 67-74Article in journal (Refereed) Published
Abstract [en]

A molecular approach was used to investigate the fungal microbiome associated with Bactrocera oleae a major key pest of Olea europea, using the ITS2 region of the ribosomal DNA (rDNA) as barcode gene. Amplicons were cloned and a representative number of sequenced fragments were used as barcode genes for the identification of fungi. The analysis of the detected sequence types (STs) enabled the identification of a total of 34 phylotypes which were associated with 10 fungal species, 3 species complexes and 8 genera. Three phylotypes remained unresolved within the order Saccharomycetales and the phylum Ascomycota because of the lack of closely related sequences in GenBank. Cladosporium was the most abundantly detected genus, followed by Alternaria and Aureobasidium, well-known components of olive sooty moulds. Interestingly, Colletotrichum sp. and other fungal plant pathogens were also detected, leading to potential new insights into heir epidemiology. (C) 2015 Elsevier Ltd and The British Mycological Society. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD , 2015. Vol. 18, p. 67-74
Keywords [en]
Cladosporium; Alternaria; Aureobasidium; Colletotrichum
National Category
Agricultural Science
Identifiers
URN: urn:nbn:se:liu:diva-147264DOI: 10.1016/j.funeco.2015.08.006OAI: oai:DiVA.org:liu-147264DiVA, id: diva2:1197419
Available from: 2018-04-12 Created: 2018-04-12 Last updated: 2018-04-24

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Citation style
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