The parasitization of honeybees by Varroa destructor represents a serious limiting factor for beekeeping. The past and current widespread use of synthetic acaricides, such as fluvalinate and coumaphos, results in the onset of pest resistance, therefore research efforts focused on the use of alternative solutions to control the population of this parasitic mite. In the present study, the efficacy of two formulates containing Oxalic Acid (OA) or Formic Acid (FA) was evaluated in real field conditions. Our results demonstrated the effectiveness of the OA formulate in controlling more than 90% of V. destructor phoretic population. On the other hand, FA shown an efficacy of 60% on phoretic mites. A similar pattern was highlighted on brood and adult bees’ infestation. The OA formulate showed a proper efficacy, highlighting its potential as alternative to chemical substances in holding the increase of V. destructor during a late summer treatment.