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An electrochemical immunosensor for cardiac Troponin I using electrospun carboxylated multi-walled carbon nanotube-whiskered nanofibres
Nanotechnology Institute, Amirkabir University of Technology, Tehran, Iran.
Textile Engineering Department, AmirKabir University of Technology, Tehran, Iran.
Biomaterials Group, Biomedical Engineering Department, Amirkabir University of Technology, Tehran, Iran.
Linköping University, Department of Physics, Chemistry and Biology, Sensor and Actuator Systems. Linköping University, Faculty of Science & Engineering.
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2018 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 182, p. 178-186Article in journal (Refereed) Published
Abstract [en]

A sandwich-type nanostructured immunosensor based on carboxylated multi-walled carbon nanotube (CMWCNT)-embedded whiskered nanofibres (WNFs) was developed for detection of cardiac Troponin I (cTnI). WNFs were directly fabricated on glassy carbon electrodes (GCE) by removing the sacrificial component (polyethylene glycol, PEG) after electrospinning of polystyrene/CMWCNT/PEG nanocomposite nanofibres, and utilised as a transducer layer for enzyme-labeled amperometric immunoassay of cTnI. The whiskered segments of CMWCNTs were activated and utilised to immobilise anti-cTnT antibodies. It was observed that the anchored CMWCNTs within the nanofibres were suitably stabilised with excellent electrochemical repeatability. A sandwich-type immuno-complex was formed between cTnI and horseradish peroxidase-conjugated anti-cTnI (HRP-anti-cTnI). The amperometric responses of the immunosensor were studied using cyclic voltammetry (CV) through an enzymatic reaction between hydrogen peroxide and HRP conjugated to the secondary antibody. The nanostructured immunosensor delivered a wide detection range for cTnI from the clinical borderline for a normal person (0.5-2 ng mL(-1)) to the concentration present in myocardial infarction patients (amp;gt; 20 ng mL(-1)), with a detection limit of similar to 0.04 ng mL(-1). It also showed good reproducibility and repeatability for three different cTnI concentration (1, 10 and 25 ng mL(-1)) with satisfactory relative standard deviations (RSD). Hence, the proposed nanostructured immunosensor shows potential for point-of-care testing.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 182, p. 178-186
Keywords [en]
Immunosensor; Cardiac Troponin I; Carbon nanotubes; Electrospinning; Whiskered nanofibres
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-147365DOI: 10.1016/j.talanta.2018.01.046ISI: 000428229200021PubMedID: 29501138Scopus ID: 2-s2.0-85042645620OAI: oai:DiVA.org:liu-147365DiVA, id: diva2:1207068
Note

Funding Agencies|Ministry of Science, Research and Technology of Iran; European Commission [PIEF-GA-2013.629251]

Available from: 2018-05-18 Created: 2018-05-18 Last updated: 2018-06-28Bibliographically approved

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The full text will be freely available from 2020-01-31 16:17
Available from 2020-01-31 16:17

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Uzun, LokmanWing Cheung, MakTURNER, APF

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