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Real-time visualizing the regulation of reactive oxygen species on Zn2+ release in cellular lysosome by a specific fluorescent probe
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, Faculty of Science & Engineering.
Anhui Univ, Peoples R China.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, Faculty of Science & Engineering.
Anhui Univ, Peoples R China.
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2018 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 264, p. 419-425Article in journal (Refereed) Published
Abstract [en]

Reactive oxygen species (ROS) regulating the release of free zinc ions (Zn2+) in cellular lysosome is closely related to various pathways of cellular signal transduction, such as inflammation and oxidative stress. Directly visualizing Zn2+ release in lysosome is essential for in-depth understanding these physiological processes, and is still an atelic challenge. In this work, we successfully fabricate a lysosome-specific Zn2+ fluorescent probe and achieve the visualization of ROS-induced Zn2+ release in lysosome of inflammatory cells. The as-prepared probe combines a green fluorophore, an ionophore with five-dentate sites, and a morpholine as the lysosome-specific localization moiety. The fluorescence of the fluorophore in the free probe is suppressed by a photoinduced electron transfer (PET) process from nitrogen atoms in the ionophore. Upon the addition of Zn2+, the fluorescence can be promoted immediately, achieving the real-time detection. Meanwhile, the probe is sensitive and selective to Zn2+, which provides the capability to detect low-concentration of free Zn2+ in lysosomes. Accordingly, the Zn2+ release was clearly observed in lysosome with the increase of ROS levels when the inflammation occurred in living cells. (c) 2018 Published by Elsevier B.V.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE SA , 2018. Vol. 264, p. 419-425
Keywords [en]
Fluorescent probe; Realtime visualization; Lysosome-specific Zn2+ probe; ROS regulation; Exogenous and endogenous Zn2+
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-147363DOI: 10.1016/j.snb.2018.02.055ISI: 000428032900050OAI: oai:DiVA.org:liu-147363DiVA, id: diva2:1207073
Note

Funding Agencies|Swedish Research Council (VR) [621-2013-5357]; National Natural Science Foundation of China [21705001, 21577098]; Swedish Government strategic faculty grant in material science (SFO, MATLIU) in Advanced Functional Materials (AFM) (VR) [5.1-2015-5959]; Knut and Alice Wallenberg Foundation (KAW) [2012.0083]; Centre in Nanoscience and Nanotechnology (CeNano)

Available from: 2018-05-18 Created: 2018-05-18 Last updated: 2018-06-05

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