liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Eventual re-vitrification or storage in liquid nitrogen vapor does not jeopardize the practical handling and transport of vitrified pig embryos
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Show others and affiliations
2018 (English)In: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 113, p. 229-236Article in journal (Refereed) Published
Abstract [en]

This study aimed (1) to evaluate the in vitro post-warming survival of porcine embryos after re vitrification and (2) to assess the efficacy of transport of embryos in dry shipper (DS) in maintaining the viability and quality of vitrified embryos for a 3-day period. Embryos at the compacted or cavitating morula (CCM) and unhatched blastocyst (UBL) stages were surgically obtained from weaned, crossbred sows. In the first experiment, more than 85% of the embryos survived an initial vitrification and warming and achieved comparable survival rates to those of their fresh counterparts. In contrast, those embryos subjected to a second vitrification and warming had clearly lower survival rates (60% and 64% for re vitrified embryos from the CCM and UBL groups, respectively) compared to the survival rates of the initial vitrification and fresh control groups (P amp;lt; 0.01). Hatching rates were similar in re-vitrified blastocysts derived from vitrified CCMs and fresh control groups (50.8% and 55.3%, respectively). However, differences (P amp;lt; 0.01) in hatching rates were recorded in re-vitrified blastocysts derived from vitrified UBLs and fresh control blastocysts (14.7% and 90.0%, respectively). In the second experiment, vitrified embryos were stored in a liquid nitrogen tank for one month. Then, the straws containing the embryos were transferred to a DS (DS group) or to another liquid nitrogen tank (control group) for an additional three days. Embryos from the DS and control groups had similar survival and hatching rates, regardless of the embryonic stage considered. The DS storage of CCMs and UBLs did not affect their development after culturing, including total cell numbers, compared to the control, although their apoptotic index was slightly higher (P amp;lt; 0.05), regardless of the developmental stage. In conclusion, although re-vitrification negatively affects embryo survival, this study demonstrated that amp;gt;60% of vitrified embryos could be successfully re-vitrified and re-warmed. The present study also showed the effectiveness of the DS for the storage of vitrified porcine CCMs and UBLs for at least three 3 days. (C) 2018 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC , 2018. Vol. 113, p. 229-236
Keywords [en]
Morulae; Blastocyst; Double vitrification; Dry shipper; Porcine
National Category
Obstetrics, Gynecology and Reproductive Medicine
Identifiers
URN: urn:nbn:se:liu:diva-147898DOI: 10.1016/j.theriogenology.2018.03.001ISI: 000431091900030PubMedID: 29567383OAI: oai:DiVA.org:liu-147898DiVA, id: diva2:1209634
Note

Funding Agencies|Ministry of Economy and Competitiveness (Madrid, Spain)/the European Regional Development Fund [AGL2015-69735-R]; Seneca Foundation, Murcia, Spain [19892/GERM/15]

Available from: 2018-05-23 Created: 2018-05-23 Last updated: 2018-06-18

Open Access in DiVA

fulltext(2739 kB)9 downloads
File information
File name FULLTEXT01.pdfFile size 2739 kBChecksum SHA-512
4668e7b2dd668712c8c80c73a0bcdb5f7adf7e6b63d750f3992d269960a007efd6331701d0e43d658ba9fc7521d6552b48b5734247ceddad73c0c509b9b4e28a
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
By organisation
Division of Children's and Women's healthFaculty of Medicine and Health Sciences
In the same journal
Theriogenology
Obstetrics, Gynecology and Reproductive Medicine

Search outside of DiVA

GoogleGoogle Scholar
Total: 9 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 63 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf