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Exogenous ascorbic acid enhances vitrification survival of porcine in vitro-developed blastocysts but fails to improve the in vitro embryo production outcomes
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain.
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2018 (English)In: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 113, p. 113-119Article in journal (Refereed) Published
Abstract [en]

In this study, the effects of addition of the antioxidant ascorbic acid (AsA) were evaluated during porcine in vitro embryo production (IVP) and vitrification. In experiment 1, the effects of AsA supplementation during IVM, IVF and IVC were evaluated, using a total of 2744 oocytes in six replicates. The IVM, IVF and embryo IVC media were supplemented or not (control) with 50 mu g/mL AsA in all possible combinations. No significant effects of AsA were detected in any of the maturation, fertilization or embryo development parameters assessed. In experiment 2, we evaluated the effects of adding AsA to vitrification-warming media on the post-warming survival and quality of blastocysts. Day-6 in vitro-produced blastocysts (N = 588) from six replicates were randomly divided in two groups, with vitrification and warming media either supplemented with 50 mu g/mL AsA (VW + group) or un-supplemented (VW- control). Addition of AsA increased (P amp;lt; 0.05) blastocyst survival rate after vitrification compared with that of VW control embryos. Vitrification and warming increased (P amp;lt; 0.05) the production of oxygen species (ROS) and reduced (P amp;lt; 0.05) the glutathione levels in blastocysts. Although VW + blastocysts displayed higher (P amp;lt; 0.05) ROS levels than those of fresh control blastocysts, the levels were lower (P amp;lt; 0.05) than those found in VW- control blastocysts. In conclusion, under the experimental conditions, supplementation of IVM/IVF/IVC media with AsA did not improve the embryo production in vitro. By contrast, the addition of AsA to chemically defined vitrification and warming media increased the survival of in vitro-produced porcine blastocysts by decreasing ROS production. (C) 2018 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC , 2018. Vol. 113, p. 113-119
Keywords [en]
Ascorbic acid; Porcine; In vitro maturation; In vitro fertilization; Vitrification
National Category
Obstetrics, Gynecology and Reproductive Medicine
Identifiers
URN: urn:nbn:se:liu:diva-147897DOI: 10.1016/j.theriogenology.2018.02.014ISI: 000431091900015PubMedID: 29477909OAI: oai:DiVA.org:liu-147897DiVA, id: diva2:1209636
Note

Funding Agencies|Ministry of Economy and Competitiveness (Madrid, Spain)/the European Regional Development Fund [AGL2015-69735-R]; Seneca Foundation, Murcia, Spain [19892/GERM/15]; Research Council FORMAS [221-2011-512, 2017-00946]; Swedish Research council VR, Stockholm, Sweden [521-2011-6553, 2015-05919]; FORSS (Forskningsradet i Sydostra Sverige), Linkoping, Sweden [473121, 745971]

Available from: 2018-05-23 Created: 2018-05-23 Last updated: 2018-06-18

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Rodriguez-Martinez, Heriberto
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