ZnO is distinguished multifunctional material that has wide applications in biochemical sensor devices. For extracellular measurements, Zinc oxide nano-rods will be deposited on conducting plastic substrate with annealing temperature 150 degrees C (ZNRP150) and silver wire with annealing temperature 250 degrees C (ZN(R)W250), for the extracellular glucose concentration determination with functionalized ZN(R)-coated biosensors. It was performed in phosphate buffer saline (PBS) over the range from 1 mu M to 10 mM and on human blood plasma. The prepared samples crystal structure and surface morphologies were characterized by XRD and field emission scanning electron microscope FESEM respectively. (C) 2018 The Authors. Published by Elsevier B.V.