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A water-soluble "turn-on" fluorescent probe for specifically imaging mitochondria viscosity in living cells
Wannan Med Coll, Peoples R China.
Anhui Univ, Peoples R China.
Chuzhou Vocat and Tech Coll, Peoples R China.
Wannan Med Coll, Peoples R China.
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2018 (English)In: Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, ISSN 1386-1425, E-ISSN 1873-3557, Vol. 203, p. 127-131Article in journal (Refereed) Published
Abstract [en]

Rational design of water-soluble probes for mitochondrial viscosity in practical biological applications remains a challenge. Herein, we described a novel hydro soluble benzothiazole salt derivative MitoSN, which exhibits specifically response and singular sensitivity to the mitochondria viscosity in living Hela cells. MitoSN displays an excellent fluorescence enhancement (ca. 35-fold) with the increase of the viscosity in the water-glycerol system. Moreover, confocal microscopy indicates that MitoSN is sensitive to the local viscosity and selectively stains mitochondria, the body of zebrafish as well. Importantly, MitoSN is capable to identify the viscosity difference of mitochondria in normal and nystatin treated Hela cells. The work provides a useful tool to monitor the changes of viscosity in the mitochondrial microenvironment. (C) 2018 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
PERGAMON-ELSEVIER SCIENCE LTD , 2018. Vol. 203, p. 127-131
Keywords [en]
Water-soluble; Mitochondrial targeting; Viscosity sensing; Benzothiazole salt; Zebrafish
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-149828DOI: 10.1016/j.saa.2018.05.121ISI: 000437816000016PubMedID: 29864636OAI: oai:DiVA.org:liu-149828DiVA, id: diva2:1236467
Note

Funding Agencies|Doctoral Startup Foundation of Wannan Medical College [wYRCQD201715]; Natural Science Foundation of Anhui Province [1508085SMB208]; Science Foundation of Department Education of Anhui Province [KJ2017ZD49]; Talent Foundation of the Education Department of Anhui [gxbjZD2016111]

Available from: 2018-08-02 Created: 2018-08-02 Last updated: 2018-08-02

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Hu, Zhang-Jun
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Molecular Surface Physics and Nano ScienceFaculty of Science & Engineering
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