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Establishment of cohesion at the pericentromere by the Ctf19 kinetochore subcomplex and the replication fork-associated factor, Csm3
The Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, United Kingdom.
The Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, United Kingdom.
2009 (English)In: PLoS Genetics, ISSN 1553-7390, E-ISSN 1553-7404, Vol. 5, no 9, article id e1000629Article in journal (Refereed) Published
Abstract [en]

The cohesin complex holds sister chromatids together from the time of their duplication in S phase until their separation during mitosis. Although cohesin is found along the length of chromosomes, it is most abundant at the centromere and surrounding region, the pericentromere. We show here that the budding yeast Ctf19 kinetochore subcomplex and the replication fork-associated factor, Csm3, are both important mediators of pericentromeric cohesion, but they act through distinct mechanisms. We show that components of the Ctf19 complex direct the increased association of cohesin with the pericentromere. In contrast, Csm3 is dispensable for cohesin enrichment in the pericentromere but is essential in ensuring its functionality in holding sister centromeres together. Consistently, cells lacking Csm3 show additive cohesion defects in combination with mutants in the Ctf19 complex. Furthermore, delaying DNA replication rescues the cohesion defect observed in cells lacking Ctf19 complex components, but not Csm3. We propose that the Ctf19 complex ensures additional loading of cohesin at centromeres prior to passage of the replication fork, thereby ensuring its incorporation into functional linkages through a process requiring Csm3.

Place, publisher, year, edition, pages
Public Library Science , 2009. Vol. 5, no 9, article id e1000629
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-150122DOI: 10.1371/journal.pgen.1000629ISI: 000270817800028PubMedID: 19730685OAI: oai:DiVA.org:liu-150122DiVA, id: diva2:1239324
Available from: 2018-08-16 Created: 2018-08-16 Last updated: 2018-08-16

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
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