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Mobile colistin resistance gene mcr-5 in porcine Aeromonas hydrophila
China Agr Univ, Peoples R China.
China Agr Univ, Peoples R China.
Publ Hlth Agcy Sweden, Sweden; Karolinska Inst, Sweden.
China Agr Univ, Peoples R China.
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2018 (English)In: Journal of Antimicrobial Chemotherapy, ISSN 0305-7453, E-ISSN 1460-2091, Vol. 73, no 7, p. 1777-1780Article in journal (Refereed) Published
Abstract [en]

Objectives: To characterize the mobile colistin resistance gene mcr-5 in Aeromonas hydrophila from backyard pigs in rural areas of China. Methods: Pig faecal samples from 194 households were directly tested for the presence of mcr-5 by PCR assay and the phenotypic antimicrobial susceptibility profiles of the mcr-5-positive isolates were determined using the broth dilution method. The genomic location and transferability of mcr-5 were analysed by S1-PFGE with Southern blotting and DNA hybridization, and natural transformation, respectively. One strain isolated from an mcr-5-positive sample was subjected to WGS and the stability of the mcr-5-harbouring plasmid over successive generations was examined by subculturing. Results: One mcr-5-positive A. hydrophila isolate showing resistance, with a colistin MIC of 4 mg/L, was isolated from a backyard pig faecal sample. mcr-5 was located on a 7915 bp plasmid designated pI064-2, which could naturally transform into a colistin-susceptible A. hydrophila strain of porcine origin and mediated colistin resistance in both the original isolate and its transformants. The plasmid backbone (3790 bp) of pI064-2 showed 81% nucleotide sequence identity to the corresponding region of the ColE2-type plasmid pAsa1 from Aeromonas salmonicida, while similar replication primases are widely distributed among aeromonads, Enterobacteriaceae and Pseudomonas species. Conclusions: To the best of our knowledge, this is the first identification of the novel colistin resistance gene mcr-5 in an A. hydrophila isolate from the faeces of a backyard pig. mcr-5 is expected to be able to disseminate among different bacterial species and genera.

Place, publisher, year, edition, pages
OXFORD UNIV PRESS , 2018. Vol. 73, no 7, p. 1777-1780
National Category
Microbiology
Identifiers
URN: urn:nbn:se:liu:diva-150293DOI: 10.1093/jac/dky110ISI: 000438375800006PubMedID: 29659855OAI: oai:DiVA.org:liu-150293DiVA, id: diva2:1239523
Note

Funding Agencies|National Natural Science Foundation of China [81361138021]; Swedish Research Council [D0879801]

Available from: 2018-08-16 Created: 2018-08-16 Last updated: 2018-08-16

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