C-type natriuretic peptide as a possible local modulator of aldosterone secretion in bovine adrenal zona glomerulosaShow others and affiliations
1996 (English)In: Endocrinology, ISSN 0013-7227, E-ISSN 1945-7170, Vol. 137, no 1, p. 42-46Article in journal (Refereed) Published
Abstract [en]
Although atrial and brain natriuretic peptides are well known to be involved in the regulation of cardiovascular and endocrine functions as circulating hormones, the roles of the C-type natriuretic peptide (CNP) remain unknown. We examined the effects of CNP on the secretion of aldosterone and cyclic nucleotides from bovine adrenal zona glomerulosa cells in culture. CNP produced a dose-dependent increase in the basal secretion of cGMP, with an EC50 of 3.8 x 10(-10)M. CNP significantly inhibited the ACTH-induced increase in aldosterone and cAMP in a dose-related manner, with an IC50 of 3.6 x 10(-10)M. Although ACTH itself did not increase cGMP secretion, the addition of CNP elicited a significant increase in cGMP secretion. The effects of CNP on the basal secretion of cGMP and the ACTH-induced secretion of aldosterone were significantly reversed by a nonpeptide natriuretic peptide receptor antagonist, HS-142-1. CNP immunoreactivity was localized in the zona glomerulosa by immunohistochemical staining. In addition, expression of CNP messenger RNA and natriuretic peptide B receptor messenger RNA was demonstrated by RT-PCR in the zona glomerulosa tissue and cells in culture. These findings suggest that CNP is a local factor regulating ACTH-induced aldosterone secretion through a guanylyl cyclase-cGMP pathway.
Place, publisher, year, edition, pages
Oxford University Press, 1996. Vol. 137, no 1, p. 42-46
National Category
Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-150406DOI: 10.1210/endo.137.1.8536640PubMedID: 8536640OAI: oai:DiVA.org:liu-150406DiVA, id: diva2:1240669
2018-08-222018-08-222018-08-30Bibliographically approved