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Immobilized enzyme film, protein immobilized film and process for forming the same: Patent abstracts. Patent No. 5356757
NEC CorporationTokyo, Japan.
NEC CorporationTokyo, Japan.
1995 (English)In: Biotechnology Advances, ISSN 0734-9750, E-ISSN 1873-1899, Vol. 13, no 2, p. 300-300Article in journal (Refereed) Published
Abstract [en]

Disclosed are an immobilized enzyme film, characterized in that said film is formed using an enzyme solution prepared by adding 1 to 3 parts by weight of a 50 to 100 wt % water-soluble crosslinking agent having at least two epoxy groups in the molecule and 1 to 3 parts by weight of a 1 to 2 wt % glutaraldehyde to 1 to 3 parts by weight of a 10 to 50 wt % aqueous protein solution containing an enzyme, and a process for forming the same on an ion-sensitive field effect transistor. The thus formed immobilized enzyme film is of an uniform thickness and stable by virtue of its increased hydrophilicity and higher elasticity, whereby deactivation of the enzyme to be caused by the shrinkage of the film can be prevented.

Place, publisher, year, edition, pages
Elsevier, 1995. Vol. 13, no 2, p. 300-300
National Category
Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-150409DOI: 10.1016/0734-9750(95)96126-6OAI: oai:DiVA.org:liu-150409DiVA, id: diva2:1240683
Available from: 2018-08-22 Created: 2018-08-22 Last updated: 2018-08-30Bibliographically approved

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Shionoya, Kiseko

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