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Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?
Univ Murcia, Spain.
Univ Murcia, Spain.
Univ Murcia, Spain.
Univ Murcia, Spain.
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2018 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 195, p. 30-37Article in journal (Refereed) Published
Abstract [en]

This study aimed to elucidate the effect of seminal plasma (SP) from post-SRF on boar sperm freezability and, in addition, to determine the relevance of sperm itself to sustain cryopreservation, regardless of the SP surrounding them. Twelve ejaculates from three boars were manually collected in fractions/portions, P1: the first 10 mL of the SRF, P2: the rest of the SRF and the post-SRF. Immediately, samples were centrifuged to separate spermatozoa from the surrounding SP. Spermatozoa from P1 and P2 were then incubated with its own SP or that from post-SRF, diluted in BTS (1:1, v/v) at 17 degrees C overnight before being frozen in 0.5 mL straws using a standard protocol. Sperm motility (total and progressive) deteriorated (P amp;lt; 0.05) when P1- or P2-sperm when incubated overnight in SP from post-SRF, while sperm viability differed between P1 and P2 (P amp;lt; 0.05) regardless of the SP they were incubated in. Post-thaw sperm quality and functionality differed between P1 and P2, regardless of the SP used for overnight pre-freezing incubation. Post-thaw motility (P amp;lt; 0.05) and viability (P amp;lt; 0.01), as well as plasma membrane fluidity (P amp;lt; 0.05) or lipid peroxidation values (P amp;lt; 0.01) were best in P1 sperm compared to those of P2. The protein profile of sperm from P1 and P2, analyzed by 2D-PAGE, showed qualitative differences, which suggest that sperm rather than SP would explain differences in sperm freezability between ejaculate fractions/portions. Use of P1 fraction spermatozoa seems thus optimal for cryopreservation.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2018. Vol. 195, p. 30-37
Keywords [en]
Sperm cryopreservation; Seminal plasma; Ejaculate portions; Sperm protein profile; Boar
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-150223DOI: 10.1016/j.anireprosci.2018.05.002ISI: 000439748200005PubMedID: 29773470OAI: oai:DiVA.org:liu-150223DiVA, id: diva2:1240748
Note

Funding Agencies|Seneca Foundation Murcia, Spain [19892/GERM-15]; Swedish Research Council Formas, Stockholm, Sweden [2017-00946]; China Scholarship Council [2013/3009]

Available from: 2018-08-22 Created: 2018-08-22 Last updated: 2018-09-19

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Rodriguez-Martinez, Heriberto
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