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Collagen-Based Fillers as Alternatives to Cyanoacrylate Glue for the Sealing of Large Corneal Perforations
Moorfields Eye Hosp NHS Fdn Trust, England; UCL Inst Ophthalmol, England; Univ Sydney, Australia.
Uppsala Univ, Sweden; LV Prasad Eye Inst, India.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Neuro and Inflammation Science. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Anaesthetics, Operations and Specialty Surgery Center, Department of Ophthalmology in Linköping.
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2018 (English)In: Cornea, ISSN 0277-3740, E-ISSN 1536-4798, Vol. 37, no 5, p. 609-616Article in journal (Refereed) Published
Abstract [en]

Purpose: To describe the use of collagen-based alternatives to cyanoacrylate glue for the sealing of acute corneal perforations. Methods: A collagen analog comprising a collagen-like peptide conjugated to polyethylene glycol (CLP-PEG) and its chemical crosslinker were tested for biocompatibility. These CLP-PEG hydrogels, which are designed to act as a framework for corneal tissue regeneration, were then tested as potential fillers in ex vivo human corneas with surgically created full-thickness perforations. Bursting pressures were measured in each of 3 methods (n = 10 for each condition) of applying a seal: 1) cyanoacrylate glue with a polyethylene patch applied ab externo (gold standard); 2) a 100-mu m thick collagen hydrogel patch applied ab interno, and 3) the same collagen hydrogel patch applied ab interno supplemented with CLP-PEG hydrogel molded in situ to fill the remaining corneal stromal defect. Results: Cyanoacrylate gluing achieved a mean bursting pressure of 325.9 mm Hg, significantly higher than the ab interno patch alone (46.3 mm Hg) and the ab interno patch with the CLP-PEG filler (86.6 mm Hg). All experimental perforations were sealed effectively using 100 mu m hydrogel sheets as an ab interno patch, whereas conventional ab externo patching with cyanoacrylate glue failed to provide a seal in 30% (3/10) cases. Conclusions: An ab interno patch system using CLP-PEG hydrogels designed to promote corneal tissue regeneration may be a viable alternative to conventional cyanoacrylate glue patching for the treatment of corneal perforation. Further experimentation and material refinement is required in advance of clinical trials.

Place, publisher, year, edition, pages
LIPPINCOTT WILLIAMS & WILKINS , 2018. Vol. 37, no 5, p. 609-616
Keywords [en]
perforation; repair; collagen; collagen-like peptide; regeneration
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:liu:diva-151217DOI: 10.1097/ICO.0000000000001459ISI: 000443068700017PubMedID: 29176452OAI: oai:DiVA.org:liu-151217DiVA, id: diva2:1248019
Note

Funding Agencies|Vinnova grant [2013-04645]; Kick Start Grant (SMS, Sydney University); Vinnova grant; Moorfields and University College London NIHR BRC in Ophthalmology

Available from: 2018-09-13 Created: 2018-09-13 Last updated: 2018-09-13

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Liszka, AnetaFagerholm, PerBuznyk, OleksiyGriffith, May
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Division of Cell BiologyFaculty of Medicine and Health SciencesDivision of Neuro and Inflammation ScienceDepartment of Ophthalmology in Linköping
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