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A Microbore Tubing Based Spiral for Multistep Cell Fractionation
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Sungkyunkwan Univ, South Korea.
Sungkyunkwan Univ, South Korea.
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2018 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 90, no 21, p. 12909-12916Article in journal (Refereed) Published
Abstract [en]

Cells were separated with the aid of a multistep spiral fractionation device, utilizing hydrodynamic forces in a spiral tubing. The spiral was fabricated using "off-the-shelf microbore tubing, allowing for cheap and fast prototyping to achieve optimal cell separation. As a first step, a model system with 20 and 40 mu m beads was used to demonstrate the effectiveness of the multistep separation device. With an initial purity of 5%, a separation purity of 83% was achieved after a two-step separation with the addition of 0.1% polyethylene glycol (PEG)-8000. Next, doxorubicinresistant polyploid giant breast cancer cells (MDA-MB-231) were separated from doxorubicin-sensitive monoploid small breast cancer cells in the same fashion as the beads, resulting in a purity of around 40%, while maintaining a cell viability of more than 90%. Combined with basic cell analytical methods, the hydrodynamic separation principle of the device could be envisaged to be useful for a variety of cell fractionation needs in cell biology and in clinical applications.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2018. Vol. 90, no 21, p. 12909-12916
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-153171DOI: 10.1021/acs.analchem.8b03532ISI: 000449722500080PubMedID: 30285432OAI: oai:DiVA.org:liu-153171DiVA, id: diva2:1267318
Note

Funding Agencies|Swedish Research Council (VR) [2016-07107]; National Research Foundation (NRF) of Korea within the Korea-Sweden Joint Research program [2016K1A3A1A47921653]

Available from: 2018-12-01 Created: 2018-12-01 Last updated: 2019-01-22

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