liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Immobilization and Interaction of Biomolecules at the Solid-Liquid Interface: Applications to Solid-Phase Analytical Techniques
Linköping University, Department of Physics, Measurement Technology, Biology and Chemistry. Linköping University, The Institute of Technology.
1986 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The development of solid-phase-analytical techniques based on surface-concentration-measuring devices requires a stable and reproducible immobilization of biomolecules on well-characterized solid surfaces. This study reports on the immobilization of Immunoglobulin G, Protein A and Fibronectin on chemically modified silica surfaces. Reactive groups were introduced to the silica surfaces by chemical-vapour deposition of silane. The silanized surfaces were characterized by ellipsometry, contact angle measurements and scanning electron microscopy, which revealed smooth, stable and reproducible silane films of monolayer character. Immobilization by adsorption as well as covalent immobilization by the use of thiol-disulphide exchange reactions, thereby controlling the maximum number of covalent bonds to the surface, were studied. The immobilization was quantitated in a study applying both ellipsometry and radiotracer techniques. The amounts of immobilized proteins obtained from the radiolabeling experiments agreed well with those calculated from ellipsometric data. The results outline the possibilities of obtaining a controlled covalent binding of biomolecules to solid surfaces with an optimal stability and biological activity of the immobilized molecules.

Place, publisher, year, edition, pages
Linköping, Sweden: Department of Physics and Measurement Technology , 1986. , p. 13
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 145
Keywords [en]
Protein immobilization, silica, Immunoglobulins, Protein A, Fibronectin, chemical-vapour deposition, silane, ellipsometry, radiolabeling, contact-angle measurement, solid-phase analytical techniques
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-153787ISBN: 9178700612 (print)OAI: oai:DiVA.org:liu-153787DiVA, id: diva2:1277386
Public defence
1986-06-03, BDL-6, Campus Valla, Linköping, Sweden, 14:00 (English)
Available from: 2019-01-10 Created: 2019-01-10 Last updated: 2019-01-10Bibliographically approved

Open Access in DiVA

No full text in DiVA

Search in DiVA

By author/editor
Jönsson, Ulf
By organisation
Department of Physics, Measurement Technology, Biology and ChemistryThe Institute of Technology
Physical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 29 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf