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Combination of Nonstructural Protein 1-Based Enzyme-Linked Immunosorbent Assays Can Detect and Distinguish Various Dengue Virus and Zika Virus Infections
Univ Hawaii Manoa, HI 96822 USA.
Univ Hawaii Manoa, HI 96822 USA.
Kaohsiung Med Univ Hosp, Taiwan; Kaohsiung Med Univ Hosp, Taiwan; Kaohsiung Med Univ, Taiwan; Kaohsiung Med Univ, Taiwan.
Linköping University, Faculty of Medicine and Health Sciences.
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2019 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 57, no 2, article id UNSP e01464-18Article in journal (Refereed) Published
Abstract [en]

The recent outbreaks of Zika virus (ZIKV) and associated birth defects in regions of dengue virus (DENV) endemicity emphasize the need for sensitive and specific serodiagnostic tests. We reported previously that enzyme-linked immunosor- bent assays (ELISAs) based on the nonstructural protein 1 (NS1) of DENV serotype 1 (DENV1) and ZIKV can distinguish primary DENV1, secondary DENV, and ZIKV infections. Whether ELISAs based on NS1 proteins of other DENV serotypes can discriminate various DENV and ZIKV infections remains unknown. We herein developed DENV2, DENV3, and DENV4 NS1 IgG ELISAs to test convalescent- and postconvalescent-phase samples from reverse transcription-PCR-confirmed cases, including 25 primary DENV1, 24 primary DENV2, 10 primary DENV3, 67 secondary DENV, 36 primary West Nile virus, 38 primary ZIKV, and 35 ZIKV with previous DENV infections as well as 55 flavivirus-naive samples. Each ELISA detected primary DENV infection with a sensitivity of 100% for the same serotype and 23.8% to 100% for different serotypes. IgG ELISA using a mixture of DENV1-4 NS1 proteins detected different primary and secondary DENV infections with a sensitivity of 95.6% and specificity of 89.5%. The ZIKV NS1 IgG ELISA detected ZIKV infection with a sensitivity of 100% and specificity of 82.9%. On the basis of the relative optical density ratio, the combination of DENV1-4 and ZIKV NS1 IgG ELISAs distinguished ZIKV with previous DENV and secondary DENV infections with a sensitivity of 91.7% to 94.1% and specificity of 87.0% to 95.0%. These findings have important applications to serodiagnosis, serosurveillance, and monitoring of both DENV and ZIKV infections in regions of endemicity.

Place, publisher, year, edition, pages
AMER SOC MICROBIOLOGY , 2019. Vol. 57, no 2, article id UNSP e01464-18
Keywords [en]
Zika virus; dengue virus; nonstructural protein 1; serodiagnosis
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-154569DOI: 10.1128/JCM.01464-18ISI: 000457592200019PubMedID: 30429254OAI: oai:DiVA.org:liu-154569DiVA, id: diva2:1290499
Note

Funding Agencies|National Institute of Allergy and Infectious Diseases, NIH [R01AI110769-01, R21AI135292-01A1, R01 AI099631, U54 AI065359, P01 AI106695, U19 AI118610]; National Institute of General Medical Sciences, NIH [P30GM114737]; Ministry of Health and Welfare, Taiwan [MOHW107-TDU-B-212-123006]; National Health Research Institute, Taiwan [NHRI-MR-107-PP-38]

Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20

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