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Nanoimmunosensor based on ZnO nanorods for ultrasensitive detection of 17 beta-Estradiol
BITS, India.
Acromed Invest AB, Sweden; COMSATS Univ, Pakistan.
BITS, India.
Acromed Invest AB, Sweden.
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2019 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 126, p. 15-22Article in journal (Refereed) Published
Abstract [en]

Advances in nanostructured materials have facilitated the development of novel sensitive techniques for detection of environmental and clinical analytes. There is immense need for development of devices that can detect analytes at concentrations as low as few pg mL(-1). The comparable size of nanostructured materials and biomolecules enabled the integration of biological systems with nanometer sized structures. Herein, we demonstrate a Zinc Oxide nanorods (ZnONRs) integrated ultrasensitive label-free biosensor with femtomolar (0.01 pg mL(-1)) sensitivity for the endocrine disruptor 17 beta-Estradiol (E2). The ZnONRs, average width 50 nm and length 325 nm, were grown on the silver electrode surface (Ag-ZnONRs). Monoclonal antibodies of E2 (mAb-E2) were covalently immobilized on ZnONRs surface and measured using electrochemical impedance spectroscopy (EIS). A linear detection range of 0.1-200 pg mL(-1) for E2 with R-2 = 0.99 and % RSD = 4.35 (n = 3, assay volume 90 mu L) was achieved for the developed nano-sensing system. A significant enhancement in the sensitivity was achieved in the presence of ZnONRs, enabling the limit of quantification down to 0.1 pg(-1) with 2.7 % capacitance change per decade. In addition, a further increase in sensitivity due to assay volume reduction (20 mu L) was observed enabling further scope of miniaturization.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY , 2019. Vol. 126, p. 15-22
Keywords [en]
Zinc oxide nanorods; Endocrine disrupting chemicals; 17 beta-Estradiol; Capacitive sensor; EIS
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-154561DOI: 10.1016/j.bios.2018.10.004ISI: 000457659500003PubMedID: 30388549OAI: oai:DiVA.org:liu-154561DiVA, id: diva2:1290510
Note

Funding Agencies|BITS, Pilani

Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20

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