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Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes
Univ Nebraska, NE 68588 USA.
Leibniz Inst Polymerforsch Dresden eV, Germany; Tech Univ Dresden, Germany.
Univ Nebraska, NE 68588 USA.
Univ Nebraska, NE 68588 USA.
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2019 (English)In: Frontiers in Chemistry, E-ISSN 2296-2646, Vol. 7, article id 51Article in journal (Refereed) Published
Abstract [en]

Substrate mediated gene delivery (SMD) is a method of immobilizing DNA complexes to a substrate via covalent attachment or nonspecific adsorption, which allows for increased transgene expression with less DNA compared to traditional bolus delivery. It may also increase cells receptivity to transfection via cell-material interactions. Substrate modifications with poly(acrylic) acid (PM) brushes may improve SMD by enhancing substrate interactions with DNA complexes via tailored surface chemistry and increasing cellular adhesion via moieties covalently bound to the brushes. Previously, we described a simple method to graft PM brushes to Ti and further demonstrated conjugation of cell adhesion peptides (i.e., RGD) to the PM brushes to improve biocompatibility. The objective of this work was to investigate the ability of Ti substrates modified with PM-RGD brushes (PM-RGD) to immobilize complexes composed of branched polyethyleneimine and DNA plasmids (bPEI-DNA) and support SMD in NIH/3T3 fibroblasts. Transfection in NIH/3T3 cells cultured on bPEI-DNA complexes immobilized onto PM-RGD substrates was measured and compared to transfection in cells cultured on control surfaces with immobilized complexes including Flat Ti, PM brushes modified with a control peptide (RGE), and unmodified PM. Transfection was two-fold higher in cells cultured on PM-RGD compared to those cultured on all control substrates. While DNA immobilization measured with radiolabeled DNA indicated that all substrates (PM-RGD, unmodified PM, Flat Ti) contained nearly equivalent amounts of loaded DNA, ellipsometric measurements showed that more total mass (i.e., DNA and bPEI, both complexed and free) was immobilized to PM and PM-RGD compared to Flat Ti. The increase in adsorbed mass may be attributed to free bPEI, which has been shown to improve transfection. Further transfection investigations showed that removing free bPEI from the immobilized complexes decreased SMD transfection and negated any differences in transfection success between cells cultured on PM-RGD and on control substrates, suggesting that free bPEI may be beneficial for SMD in cells cultured on bPEI-DNA complexes immobilized on PM-RGD grafted to Ti. This work demonstrates that substrate modification with PM-RGD is a feasible method to enhance SMD outcomes on Ti and may be used for future applications such as tissue engineering, gene therapy, and diagnostics.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA , 2019. Vol. 7, article id 51
Keywords [en]
substrate mediated; polymer brushes; poly(acrylic) acid; nonviral gene delivery; branched polyethylenimine; RGD ligand
National Category
Other Basic Medicine
Identifiers
URN: urn:nbn:se:liu:diva-154549DOI: 10.3389/fchem.2019.00051ISI: 000458035200001OAI: oai:DiVA.org:liu-154549DiVA, id: diva2:1290525
Note

Funding Agencies|Nebraska Research Initiative,; University of Nebraska-Lincoln Tobacco Settlement Funds; National Institutes of Health [1 DP2 EB025760-01]; Leibniz Institute of Polymer Research Dresden; National Science Foundation (CAREER) [CBET1254415]; Center for Nanohybrid Functional Materials [NSF EPS-1004094]; J.A. Woollam Company; USDA CSREES-Nebraska [NEB-21-146]

Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-03-15

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