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High performance electrochemical biosensor based on 3D nitrogen-doped reduced graphene oxide electrode and tetrahedral DNA nanostructure
Tsinghua Peking Joint Ctr Life Sci, Peoples R China.
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics. Linköping University, Faculty of Science & Engineering. Nanjing Tech Univ, Peoples R China.
Chinese Acad Sci, Peoples R China.
Peking Univ, Peoples R China.
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2019 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 194, p. 273-281Article in journal (Refereed) Published
Abstract [en]

In this study, an electrochemical biosensor was developed for highly sensitive and specific detection of target miRNA-155. The structure was formed by the hybridization of a tetrahedral DNA nanostructure-based biomolecular probe assembled on 3D nitrogen-doped reduced graphene oxide/gold nanoparticles (3D N-doped rGO/AuNPs) electrode surface. Upon addition of target miRNA-155, the gold and silver nanorod/thionine/complementary DNA (AuAgNR/Thi/F) was hybridized with the target, and used for signal amplification, catalyzing the reduction of Thi as an electron mediator. Due to the signal amplification by the enhanced immobilization of DNA on the surface of 3D N-doped rGO/AuNPs electrode and AuAgNR/Thi, coupling the low background signal produced by blank solution, electrochemical performance of the device was optimized to be proportional to miRNA-155 concentration in the range of 1 x 10(-11) to 1 x 10(-4) M with a detection limit of 1 x 10(-12) M. In addition, direct detection in serum is demonstrated with high specificity. Thus, this biosensor is potentially applicable for microRNA detection in medical research and early clinical diagnosis.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2019. Vol. 194, p. 273-281
Keywords [en]
3D N-doped rGO/AuNPs; MicroRNA; AuAgNR; Tetrahedral DNA; Electrochemical biosensor
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:liu:diva-154541DOI: 10.1016/j.talanta.2018.09.110ISI: 000456899700036PubMedID: 30609531OAI: oai:DiVA.org:liu-154541DiVA, id: diva2:1290530
Note

Funding Agencies|National Natural Science Foundation of China [2127369, 61704078]; Tsinghua-Peking Joint Center for Life Sciences post-doctoral fund; Natural Science Foundation of Jiangsu Province of China [BK20160990]

Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-03-26

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The full text will be freely available from 2020-10-09 12:20
Available from 2020-10-09 12:20

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