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The proteome of frozen-thawed pig spermatozoa is dependent on the ejaculate fraction source
Univ Murcia, Spain.
Univ Murcia, Spain.
Univ Murcia, Spain.
Univ Murcia, Spain.
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2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 705Article in journal (Refereed) Published
Abstract [en]

The preservation of sperm functional parameters and fertility post-cryopreservation largely varies in the porcine, a species with a fractionated ejaculate. Although intrinsic individual differences have primarily been linked to this variation, differences in protein abundance among frozen-thawed (FT)-spermatozoa are far more relevant. This study, performed in two experiments, looked for proteomic quantitative differences between FT-sperm samples differing in post-thaw viability, motility, apoptosis, membrane lipid peroxidation and nuclear DNA fragmentation. The spermatozoa were either derived from the sperm-rich ejaculate fraction (SRF) or the entire ejaculate (Experiment 1) or from the first 10 mL of the SRF, the remaining SRF and the post-SRF (Experiment 2). Quantitative sperm proteomic differences were analysed using a LC-ESI-MS/MS-based SWATH approach. In Experiment 1, FT-spermatozoa from the SRF showed better preservation parameters than those from the entire ejaculate, with 26 Sus scrofa proteins with functional sperm relevance showing relative quantitative differences (FC amp;gt;= 1.5) between sperm sources. In Experiment 2, FT-spermatozoa from the first 10 mL of the SRF and the remaining SRF were qualitatively better than those from the post-SRF, and 187 proteins showed relative quantitative differences among the three ejaculate sources. The results indicate that quantitative proteome differences are linked to sperm cryosurvival.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2019. Vol. 9, article id 705
National Category
Bioinformatics and Systems Biology
Identifiers
URN: urn:nbn:se:liu:diva-154691DOI: 10.1038/s41598-018-36624-5ISI: 000456554600144PubMedID: 30679492OAI: oai:DiVA.org:liu-154691DiVA, id: diva2:1292645
Note

Funding Agencies|MINECO (Spain) [AGL2015-69738-R]; FEDER funds (EU) [AGL2015-69738-R]; Seneca Foundation Murcia, Spain [19892/GERM-15]; FORSS, Stockholm, Sweden [745971]; Swedish Research Council FORMAS, Stockholm, Sweden [2017-00946]; Seneca Foundation (Murcia, Spain); MECD (Madrid, Spain); China Scholarship Council

Available from: 2019-02-28 Created: 2019-02-28 Last updated: 2019-03-15

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Rodriguez-Martinez, Heriberto
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Division of Children's and Women's healthFaculty of Medicine and Health Sciences
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