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Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation
Anhui Univ, Peoples R China; Anhui Univ, Peoples R China.
Anhui Univ, Peoples R China; Anhui Univ, Peoples R China.
Anhui Univ, Peoples R China; Anhui Univ, Peoples R China.
Anhui Univ, Peoples R China; Anhui Univ, Peoples R China.
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2019 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 58, no 8, p. 2261-2265Article in journal (Refereed) Published
Abstract [en]

Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the regulation of enzyme activity via gasotransmitters in situ remains a long-standing challenge. Herein, we report a three-channel imaging correlation by a single dual-reactive fluorescent probe to measure the dependence of phosphatase activity on the H2S level in cells. The two sites of the probe reactive to H2S and phosphatase individually produce blue and green fluorescent responses, respectively, and resonance energy transfer can be triggered by their coexistence. Fluorescent analysis based on the three-channel imaging correlation shows that cells have an ideal level of H2S to promote phosphatase activity up to its maximum. Significantly, a slight deviation from this H2S level leads to a sharp decrease of phosphatase activity. The discovery further strengthens our understanding of the importance of H2S in cellular signaling and in various human diseases.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH , 2019. Vol. 58, no 8, p. 2261-2265
Keywords [en]
enzyme regulation; fluorescent probes; gasotransmitters; imaging agents; phosphatase
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:liu:diva-154661DOI: 10.1002/anie.201811391ISI: 000458417700011PubMedID: 30589211OAI: oai:DiVA.org:liu-154661DiVA, id: diva2:1293221
Note

Funding Agencies|National Basic Research Program of China [2015CB932002]; National Natural Science Foundation of China [21335006, 21475135, 21375131, 21775001, 21705001, 21602003]; Anhui Provincial Natural Science Foundation of China [1708085MC68, 1808085MB32]; Hefei Physical Science Center Foundation [2017FXZY001]

Available from: 2019-03-04 Created: 2019-03-04 Last updated: 2019-03-26

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The full text will be freely available from 2019-12-27 12:53
Available from 2019-12-27 12:53

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Hu, Zhang-Jun
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Molecular Surface Physics and Nano ScienceFaculty of Science & Engineering
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