liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A mechanism for temporary bioadhesion
Univ Innsbruck, Austria.
Univ Innsbruck, Austria; Univ Mons, Belgium.
Univ Innsbruck, Austria.
Univ Innsbruck, Austria.
Show others and affiliations
2019 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 116, no 10, p. 4297-4306Article in journal (Refereed) Published
Abstract [en]

The flatworm Macrostomum lignano features a duo-gland adhesive system that allows it to repeatedly attach to and release from substrates in seawater within a minute. However, little is known about the molecules involved in this temporary adhesion. In this study, we show that the attachment of M. lignano relies on the secretion of two large adhesive proteins, M. lignano adhesion protein 1 (Mlig-ap1) and Mlig-ap2. We revealed that both proteins are expressed in the adhesive gland cells and that their distribution within the adhesive footprints was spatially restricted. RNA interference knockdown experiments demonstrated the essential function of these two proteins in flatworm adhesion. Negatively charged modified sugars in the surrounding water inhibited flatworm attachment, while positively charged molecules impeded detachment. In addition, we found that M. lignano could not adhere to strongly hydrated surfaces. We propose an attachment-release model where Mlig-ap2 attaches to the substrate and Mlig-ap1 exhibits a cohesive function. A small negatively charged molecule is secreted that interferes with Mlig-ap1, inducing detachment. These findings are of relevance for fundamental adhesion science and efforts to mitigate biofouling. Further, this model of flatworm temporary adhesion may serve as the starting point for the development of synthetic reversible adhesion systems for medicinal and industrial applications.

Place, publisher, year, edition, pages
NATL ACAD SCIENCES , 2019. Vol. 116, no 10, p. 4297-4306
Keywords [en]
flatworms; bioadhesion; Platyhelminthes; bioadhesive; detachment
National Category
Biophysics
Identifiers
URN: urn:nbn:se:liu:diva-155532DOI: 10.1073/pnas.1814230116ISI: 000460242100059PubMedID: 30782790OAI: oai:DiVA.org:liu-155532DiVA, id: diva2:1299550
Note

Funding Agencies|Austrian Science Fund [P 25404-B25, P 30347, J 4071]; University of Innsbruck; Autonome Provinz Bozen; Austrian Academy of Sciences OAW; Tyrolian Science Fund TWF; European Cooperation in Science and Technology (COST) Actions [TD0906, CA15216]

Available from: 2019-03-27 Created: 2019-03-27 Last updated: 2019-03-27

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Ederth, Thomas
By organisation
Molecular PhysicsFaculty of Science & Engineering
In the same journal
Proceedings of the National Academy of Sciences of the United States of America
Biophysics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 138 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf