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In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells
Section of Immunity, Infection and Inflammation, School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, UK.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. Department of Molecular Medicine and Gene Therapy, Lund Stem Cell CenterLund University, Lund, Sweden.
2018 (English)In: Cancer Stem Cells: Methods and Protocols / [ed] Gianpaolo Papaccio and Vincenzo Desiderio, New York: Humana Press, 2018, Vol. 1692, p. 89-95Chapter in book (Refereed)
Abstract [en]

Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.

Place, publisher, year, edition, pages
New York: Humana Press, 2018. Vol. 1692, p. 89-95
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1692
Keywords [en]
Agarose; Cancer stem cell; Clonogenic assay; Colony growth; Crystal violet
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-152541DOI: 10.1007/978-1-4939-7401-6_8PubMedID: 28986889ISBN: 9781493974009 (print)ISBN: 9781493974016 (electronic)OAI: oai:DiVA.org:liu-152541DiVA, id: diva2:1299649
Available from: 2019-03-28 Created: 2019-03-28 Last updated: 2019-03-29Bibliographically approved

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Jain, Mayur Vilas

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CiteExportLink to record
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Citation style
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