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Luminescent-Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-5582-140X
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering. Department of Physics, The Norwegian University of Science and Technology, 7491, Trondheim, Norway.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0001-5827-3587
2018 (English)In: Amyloid Proteins: Methods and Protocols / [ed] Einar M. Sigurdsson, Miguel Calero and María Gasset, Humana Press, 2018, Vol. 1779, p. 485-496Chapter in book (Refereed)
Abstract [en]

Luminescent-conjugated oligo- and polythiophenes (LCOs and LCPs) are valuable tools for optical imaging of a plethora of protein aggregates associated with amyloidoses. Here, we outline updated protocols for the application of the anionic pentameric LCO, p-FTAA, for staining and hyperspectral imaging of protein aggregates in a variety of settings such as in vitro formed amyloid fibrils, ex vivo tissue sections, and whole brain Drosophila.

Place, publisher, year, edition, pages
Humana Press, 2018. Vol. 1779, p. 485-496
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1779
Keywords [en]
Amyloid; Fluorescence imaging; Luminescent-conjugated oligothiophenes; Protein aggregates; p-FTAA
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:liu:diva-152516DOI: 10.1007/978-1-4939-7816-8_30PubMedID: 29886552ISBN: 9781493978151 (print)ISBN: 9781493978168 (electronic)OAI: oai:DiVA.org:liu-152516DiVA, id: diva2:1299744
Available from: 2019-03-28 Created: 2019-03-28 Last updated: 2019-03-29

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Nilsson, PeterLindgren, MikaelHammarström, Per

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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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