liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Imaging Extracellular Calcium in Endolymph
Columbia Univ, NY 10027 USA.
Linköping University, Department of Clinical and Experimental Medicine, Divison of Neurobiology. Linköping University, Faculty of Medicine and Health Sciences.
2018 (English)In: TO THE EAR AND BACK AGAIN - ADVANCES IN AUDITORY BIOPHYSICS, AMER INST PHYSICS , 2018, Vol. 1965, article id UNSP 080005Conference paper, Published paper (Refereed)
Abstract [en]

Hair cell mechanoelectrical transduction and adaptation are believed to be regulated by extracellular calcium. However, the majority of experiments addressing calciums role have been performed on reduced preparations in conditions that do not mimic those present in vivo. We used confocal microscopy and a low affinity (k(d) similar to 11 mu M) ratiometric fluorescent indicator to measure the extracellular calcium concentration in scala media in an in vitro preparation of the guinea pig cochlea. Microelectrodes were used to measure the cochlear microphonic potential during acoustic stimulation. The mean calcium concentration is significantly higher in the tectorial membrane (TM) than the surrounding endolymph, suggesting that the membrane acts as a calcium sink. We also observe calcium hot spots along the underside of the TM, near the outer hair cell bundles and near Hensens stripe close to the inner hair cell bundle. This suggests that the local calcium concentration near the hair bundles exceeds 100 mu M, significantly higher than the bulk endolymph. These results were corroborated with fluorescence correlation spectroscopy using a second calcium sensitive dye, Oregon Green 488-BAPTA. Following a brief exposure to loud sound, TM calcium drops dramatically and shows recovery on a similar timescale as the microphonic potential. Our results suggest that the extracellular calcium concentration near the hair bundles is much higher than previously believed and may also serve as a partial control parameter for temporary threshold shifts.

Place, publisher, year, edition, pages
AMER INST PHYSICS , 2018. Vol. 1965, article id UNSP 080005
Series
AIP Conference Proceedings, ISSN 0094-243X
National Category
Biophysics
Identifiers
URN: urn:nbn:se:liu:diva-155957DOI: 10.1063/1.5038489ISI: 000461049900042ISBN: 978-0-7354-1670-3 (print)OAI: oai:DiVA.org:liu-155957DiVA, id: diva2:1301282
Conference
13th Mechanics of Hearing Workshop (MoH) on To Ear and Back Again - Advances in Auditory Biophysics
Note

Funding Agencies|Swedish Research Council; Wenner-Gren Followship

Available from: 2019-04-01 Created: 2019-04-01 Last updated: 2019-04-01

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Search in DiVA

By author/editor
Fridberger, Anders
By organisation
Divison of NeurobiologyFaculty of Medicine and Health Sciences
Biophysics

Search outside of DiVA

GoogleGoogle Scholar

doi
isbn
urn-nbn

Altmetric score

doi
isbn
urn-nbn
Total: 2 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf