liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Retention of EsxA in the Capsule-Like Layer of Mycobacterium tuberculosis Is Associated with Cytotoxicity and Is Counteracted by Lung Surfactant
Linköping University, Department of Clinical and Experimental Medicine, Division of Neuro and Inflammation Science. Linköping University, Faculty of Medicine and Health Sciences.
Division of Nanoscopy, The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, The Netherlands.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology, Infection and Inflammation. Linköping University, Faculty of Medicine and Health Sciences.
Division of Nanoscopy, The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, The Netherlands.
Show others and affiliations
2019 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 87, no 3, article id e00803-18Article in journal (Refereed) Published
Abstract [en]

Mycobacterium tuberculosis, the pathogen that causes tuberculosis, primarily infects macrophages but withstands the host cells bactericidal effects. EsxA, also called virulence factor 6-kDa early secretory antigenic target (ESAT-6), is involved in phagosomal rupture and cell death. We provide confocal and electron microscopy data showing that M. tuberculosis bacteria grown without detergent retain EsxA on their surface. Lung surfactant has detergent-like properties and effectively strips off this surface-associated EsxA, which advocates a novel mechanism of lung surfactant-mediated defense against pathogens. Upon challenge of human macrophages with these M. tuberculosis bacilli, the amount of surface-associated EsxA rapidly declines in a phagocytosis-independent manner. Furthermore, M. tuberculosis bacteria cultivated under exclusion of detergent exert potent cytotoxic activity associated with bacterial growth. Together, this study suggests that the surface retention of EsxA contributes to the cytotoxicity of M. tuberculosis and highlights how cultivation conditions affect the experimental outcome.

Place, publisher, year, edition, pages
Washington, DC, United States: American Society for Microbiology , 2019. Vol. 87, no 3, article id e00803-18
Keywords [en]
ESAT-6; EsxA; Mycobacterium tuberculosis; cytotoxicity; human macrophage; lung surfactant
National Category
Immunology
Identifiers
URN: urn:nbn:se:liu:diva-156206DOI: 10.1128/IAI.00803-18ISI: 000462158100011PubMedID: 30602503Scopus ID: 2-s2.0-85061988279OAI: oai:DiVA.org:liu-156206DiVA, id: diva2:1303225
Note

Funding Agencies|ZON-MW MKMD program (The Netherlands Organisation for Health Research and Development); Swedish Research Council/Swedish International Cooperation Agency (SIDA) [2012-3349, 2015-02593]; Swedish Heart Lung/the Oskar II Jubilee Foundations [20130685, 20150709]; County Council of Ostergotland

Available from: 2019-04-09 Created: 2019-04-09 Last updated: 2019-09-23Bibliographically approved

Open Access in DiVA

fulltext(1249 kB)90 downloads
File information
File name FULLTEXT01.pdfFile size 1249 kBChecksum SHA-512
b20190914a59ba11f3f38ccb05c9f29a35d03af5393fa5ab7112c8541d796cc2ccfcd95261600e9e12f97655ad7f33bcb6d1319ada94b1c06610a309ff7262bf
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Raffetseder, JohannaLoitto, VesaLerm, Maria
By organisation
Division of Neuro and Inflammation ScienceFaculty of Medicine and Health SciencesDivision of Microbiology, Infection and Inflammation
In the same journal
Infection and Immunity
Immunology

Search outside of DiVA

GoogleGoogle Scholar
Total: 90 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 143 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf