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In situ microscopy as online tool for detecting microbial contaminations in cell culture
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0001-9711-794X
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Faculty of Science & Engineering.
Leibniz Univ Hannover, Germany.
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2019 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 296, p. 53-60Article in journal (Refereed) Published
Abstract [en]

Microbial contamination in mammalian cell cultures causing rejected batches is costly and highly unwanted. Most methods for detecting a contamination are time-consuming and require extensive off-line sampling. To circumvent these efforts and provide a more convenient alternative, we used an online in situ microscope to estimate the cell diameter of the cellular species in the culture to distinguish mammalian cells from microbial cells depending on their size. A warning system was set up to alert the operator if microbial cells were present in the culture. Hybridoma cells were cultured and infected with either Candida utilis or Pichia stipitis as contaminant. The warning system could successfully detect the introduced contamination and alert the operator. The results suggest that in situ microscopy could be used as an efficient online tool for early detection of contaminations in cell cultures.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2019. Vol. 296, p. 53-60
Keywords [en]
Image analysis; Mammalian cell culture; Infection; Hybridoma cells; Candida; Pichia
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-156378DOI: 10.1016/j.jbiotec.2019.03.011ISI: 000462999100007PubMedID: 30898686OAI: oai:DiVA.org:liu-156378DiVA, id: diva2:1305816
Note

Funding Agencies|Technical Faculty of Linkoping University; German Federal Ministry of Education and Research (BMBF)

Available from: 2019-04-18 Created: 2019-04-18 Last updated: 2019-04-18

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Gustavsson, RobertMandenius, Carl-FredrikLöfgren, S.
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