liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The Effect of Enzymatic Digestion on Cultured Epithelial Autografts
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Anaesthetics, Operations and Specialty Surgery Center, Department of Hand and Plastic Surgery.
Linköping University, Department of Physics, Chemistry and Biology, Molecular Physics. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-7001-9415
Show others and affiliations
2019 (English)In: Cell Transplantation, ISSN 0963-6897, E-ISSN 1555-3892, Vol. 28, no 5, p. 638-644Article in journal (Refereed) Published
Abstract [en]

Severe burns are often treated by means of autologous skin grafts, preferably following early excision of the burnt tissue. In the case of, for example, a large surface trauma, autologous skin cells can be expanded in vitro prior to transplantation to facilitate the treatment when insufficient uninjured skin is a limitation. In this study we have analyzed the impact of the enzyme (trypsin or accutase) used for cell dissociation and the incubation time on cell viability and expansion potential, as well as expression of cell surface markers indicative of stemness. Skin was collected from five individuals undergoing abdominal reduction surgery and the epidermal compartment was digested in either trypsin or accutase. Trypsin generally generated more cells than accutase and with higher viability; however, after 7 days of subsequent culture, accutase-digested samples tended to have a higher cell count than trypsin, although the differences were not significant. No significant difference was found between the enzymes in median fluorescence intensity of the analyzed stem cell markers; however, accutase digestion generated significantly higher levels of CD117- and CD49f-positive cells, but only in the 5 h digestion group. In conclusion, digestion time appeared to affect the isolated cells more than the choice of enzyme.

Place, publisher, year, edition, pages
Sage Publications, 2019. Vol. 28, no 5, p. 638-644
Keywords [en]
autografts, cell culture, epithelial cells, keratinocytes, stem cells
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:liu:diva-156422DOI: 10.1177/0963689719833305ISI: 000477971000013PubMedID: 30983404OAI: oai:DiVA.org:liu-156422DiVA, id: diva2:1306059
Note

Funding agencies: Integrative Regenerative Medicine (IGEN) center at Linkoping University

Available from: 2019-04-23 Created: 2019-04-23 Last updated: 2019-08-19

Open Access in DiVA

fulltext(445 kB)142 downloads
File information
File name FULLTEXT01.pdfFile size 445 kBChecksum SHA-512
8e11abe23335eea7fd47bd7752863d19e523cbe9b78f446c6446b3d7aeb7c5340b6f4b04beaf8557aaa9e3006f71a06566fc10e5f24327dd56c790e586cb025a
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Skog, MårtenSteinvall, IngridAili, DanielSjöberg, FolkeElmasry, Moustafa

Search in DiVA

By author/editor
Skog, MårtenSivlér, PetterSteinvall, IngridAili, DanielSjöberg, FolkeElmasry, Moustafa
By organisation
Molecular PhysicsFaculty of Science & EngineeringDivision of Surgery, Orthopedics and OncologyFaculty of Medicine and Health SciencesDepartment of Hand and Plastic Surgery
In the same journal
Cell Transplantation
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 142 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 157 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf