Depletion of cytotoxic (CD8+) T cells impairs implant fixation in rat cancellous boneShow others and affiliations
2019 (English)In: Journal of Orthopaedic Research, ISSN 0736-0266, E-ISSN 1554-527X, Vol. 37, no 4, p. 805-811Article in journal (Refereed) Published
Abstract [en]
As cytotoxic (CD8(+)) T cells seem to impair shaft fracture healing, we hypothesized that depletion of CD8(+) cells would instead improve healing of cancellous bone. Additionally, we also tested if CD8-depletion would influence the healing of ruptured Achilles tendons. Rats received a single injection of either anti-CD8 antibodies or saline and put through surgery 24 h later. Three different surgical interventions were performed as follows: (1) a drill hole in the proximal tibia with microCT (BV/TV) to assess bone formation; (2) a screw in the proximal tibia with mechanical evaluation (pull-out force) to assess fracture healing; (3) Achilles tendon transection with mechanical evaluation (force-at-failure) to assess tendon healing. Furthermore, CD8-depletion was confirmed with flow cytometry on peripheral blood. Flow cytometric analysis confirmed depletion of CD8(+) cells (p amp;lt; 0.001). Contrary to our hypothesis, depletion of CD8(+) cells reduced the implant pull-out force by 19% (p amp;lt; 0.05) and stiffness by 34% (p amp;lt; 0.01), although the bone formation in the drill holes was the same as in the controls. Tendon healing was unaffected by CD8-depletion. Our results suggest that CD8(+) cells have an important part in cancellous bone healing.
Place, publisher, year, edition, pages
John Wiley & Sons, 2019. Vol. 37, no 4, p. 805-811
Keywords [en]
bone healing; cancellous; tendon healing; cytotoxic T cells; CD8 depletion
National Category
Orthopaedics
Identifiers
URN: urn:nbn:se:liu:diva-157559DOI: 10.1002/jor.24246ISI: 000467082100001PubMedID: 30737834Scopus ID: 2-s2.0-85062344231OAI: oai:DiVA.org:liu-157559DiVA, id: diva2:1328649
Note
Funding Agencies|Swedish Research Council [2031-47-5]; AFA insurance company EU 159 7th framework program [FP7/2007-2013, 279239]; Linkoping 160 University
2019-06-222019-06-222019-06-25Bibliographically approved