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Depletion of cytotoxic (CD8+) T cells impairs implant fixation in rat cancellous bone
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Haematology.
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences.
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2019 (English)In: Journal of Orthopaedic Research, ISSN 0736-0266, E-ISSN 1554-527X, Vol. 37, no 4, p. 805-811Article in journal (Refereed) Published
Abstract [en]

As cytotoxic (CD8(+)) T cells seem to impair shaft fracture healing, we hypothesized that depletion of CD8(+) cells would instead improve healing of cancellous bone. Additionally, we also tested if CD8-depletion would influence the healing of ruptured Achilles tendons. Rats received a single injection of either anti-CD8 antibodies or saline and put through surgery 24 h later. Three different surgical interventions were performed as follows: (1) a drill hole in the proximal tibia with microCT (BV/TV) to assess bone formation; (2) a screw in the proximal tibia with mechanical evaluation (pull-out force) to assess fracture healing; (3) Achilles tendon transection with mechanical evaluation (force-at-failure) to assess tendon healing. Furthermore, CD8-depletion was confirmed with flow cytometry on peripheral blood. Flow cytometric analysis confirmed depletion of CD8(+) cells (p amp;lt; 0.001). Contrary to our hypothesis, depletion of CD8(+) cells reduced the implant pull-out force by 19% (p amp;lt; 0.05) and stiffness by 34% (p amp;lt; 0.01), although the bone formation in the drill holes was the same as in the controls. Tendon healing was unaffected by CD8-depletion. Our results suggest that CD8(+) cells have an important part in cancellous bone healing.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019. Vol. 37, no 4, p. 805-811
Keywords [en]
bone healing; cancellous; tendon healing; cytotoxic T cells; CD8 depletion
National Category
Orthopaedics
Identifiers
URN: urn:nbn:se:liu:diva-157559DOI: 10.1002/jor.24246ISI: 000467082100001PubMedID: 30737834Scopus ID: 2-s2.0-85062344231OAI: oai:DiVA.org:liu-157559DiVA, id: diva2:1328649
Note

Funding Agencies|Swedish Research Council [2031-47-5]; AFA insurance company EU 159 7th framework program [FP7/2007-2013, 279239]; Linkoping 160 University

Available from: 2019-06-22 Created: 2019-06-22 Last updated: 2019-06-25Bibliographically approved

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Bernhardsson, MagnusDietrich, FrancieleTätting, LoveEliasson, PernillaAspenberg, Per

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Bernhardsson, MagnusDietrich, FrancieleTätting, LoveEliasson, PernillaAspenberg, Per
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Division of Surgery, Orthopedics and OncologyFaculty of Medicine and Health SciencesDepartment of HaematologyDepartment of Orthopaedics in Linköping
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Journal of Orthopaedic Research
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