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Serum-responsive expression of carbonyl-metabolizing enzymes in normal and transformed human buccal keratinocytes
Karolinska Institute.
Karolinska Institute.
Linköping University, Department of Clinical and Experimental Medicine, Oto-Rhiono-Laryngology and Head & Neck Surgery . Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Reconstruction Centre, Department of ENT - Head and Neck Surgery UHL.
Karolinska Institute.
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2008 (English)In: Cellular and Molecular Life Sciences (CMLS), ISSN 1420-682X, Vol. 65, no 22, 3653-3663 p.Article in journal (Refereed) Published
Abstract [en]

Gene expression of carbonyl-metabolizing enzymes (CMEs) was investigated in normal buccal keratinocytes (NBK) and the transformed buccal keratinocyte lines SVpgC2a and SqCC/Y1. Studies were performed at a serum concentration known to induce terminal squamous differentiation (TSD) in normal cells. Overall, 39 of 58 evaluated CMEs were found to be expressed at the transcript level. Together the transformed cell lines showed altered transcription of eight CME genes compared to NBK, substantiating earlier results. Serum increased transcript levels of ALDH1A3, DHRS3, HPGD and AKR1A1, and decreased those of ALDH4A1 in NBK; of these, the transformed, TSD-deficient cell lines partly retained regulation of ALDH1A3 and DHRS3. Activity measurements in crude cell lysates, including relevant enzymatic inhibitors, indicated significant capacity for CME-mediated xenobiotic metabolism among the cell lines, notably with an increase in serum-differentiated NBK. The results constitute the first evidence for differential CME gene expression and activity in non-differentiated and differentiated states of epithelial cells.

Place, publisher, year, edition, pages
2008. Vol. 65, no 22, 3653-3663 p.
Keyword [en]
Buccal mucosa, carbonyl-metabolizing enzymes, microarray, quinone metabolism, serum effects, terminal squamous differentiation, xenobiotic metabolism
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-16167DOI: 10.1007/s00018-008-8554-8OAI: diva2:133257
Available from: 2009-01-08 Created: 2009-01-07 Last updated: 2009-08-21

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Roberg, Karin
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Oto-Rhiono-Laryngology and Head & Neck Surgery Faculty of Health SciencesDepartment of ENT - Head and Neck Surgery UHL
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