liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Genomic and Functional Analysis of Emerging Virulent and Multidrug-Resistant Escherichia coli Lineage Sequence Type 648
Ernst Moritz Arndt Univ Greifswald, Germany; Free Univ Berlin, Germany.
Robert Koch Inst, Germany.
Robert Koch Inst, Germany.
Univ Adelaide, Australia.
Show others and affiliations
2019 (English)In: Antimicrobial Agents and Chemotherapy, ISSN 0066-4804, E-ISSN 1098-6596, Vol. 63, no 6, article id e00243-19Article in journal (Refereed) Published
Abstract [en]

The pathogenic extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global ST648 collection from various hosts (87 whole-genome sequences) combining core and accessory genomics with functional analyses and in vivo experiments suggests that ST648 is a nascent and generalist lineage, lacking clear phylogeographic and host association signals. By including large numbers of ST131 (n = 107) and ST10 (n = 96) strains for comparative genomics and phenotypic analysis, we demonstrate that the combination of multidrug resistance and high-level virulence are the hallmarks of ST648, similar to international high-risk clonal lineage ST131. Specifically, our in silico, in vitro, and in vivo results demonstrate that ST648 is well equipped with biofilm-associated features, while ST131 shows sophisticated signatures indicative of adaption to urinary tract infection, potentially conveying individual ecological niche adaptation. In addition, we used a recently developed NFDS (negative frequency-dependent selection) population model suggesting that ST648 will increase significantly in frequency as a cause of bacteremia within the next few years. Also, ESBL plasmids impacting biofilm formation aided in shaping and maintaining ST648 strains to successfully emerge worldwide across different ecologies. Our study contributes to understanding what factors drive the evolution and spread of emerging international high-risk clonal lineages.

Place, publisher, year, edition, pages
AMER SOC MICROBIOLOGY , 2019. Vol. 63, no 6, article id e00243-19
Keywords [en]
ESBL-producing clonal lineages; MDR; NFDS modeling; ST648; biofilm formation; phylogenetics; virulence
National Category
Microbiology
Identifiers
URN: urn:nbn:se:liu:diva-158307DOI: 10.1128/AAC.00243-19ISI: 000468935100023PubMedID: 30885899OAI: oai:DiVA.org:liu-158307DiVA, id: diva2:1333952
Note

Funding Agencies|German Research Foundation (DFG) [GU 1283/3-1, EW116/2-1]; Feodor Lynen Research Fellowship by the Alexander von Humboldt Foundation, Germany; Wellcome Trust Sanger Institute (Cambridge, United Kingdom) [2433ILB]; project CEITEC 2020 from the Ministry of Education, Youth, and Sports of the Czech Republic [LQ1601]

Available from: 2019-07-02 Created: 2019-07-02 Last updated: 2019-07-02

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Bonnedahl, Jonas
By organisation
Division of Microbiology, Infection and InflammationFaculty of Medicine and Health Sciences
In the same journal
Antimicrobial Agents and Chemotherapy
Microbiology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 6 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf