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Poor in vitro induction of FOXP3 and ICOS in type 1 cytokine environment activated T-cells from children with type 1 diabetes
Department of Viral Diseases and Immunology, National Public Health Institute, Helsinki, Finland.
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics. Linköping University, Faculty of Health Sciences.
Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland and Department of Paediatrics, Tampere University Hospital, Tampere, Finland.
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics. Linköping University, Faculty of Health Sciences.
2008 (English)In: Diabetes/Metabolism Research Reviews, ISSN 1520-7552, E-ISSN 1520-7560, Vol. 24, no 8, 635-641 p.Article in journal (Refereed) Published
Abstract [en]

Background

Type 1 diabetes (T1D) is characterised by loss of tolerance to beta-cell antigens, and the insulin-producing beta-cells in the pancreatic islets are destroyed by the host's own immune system. Immunological risk factors associated with T1D are related to the defects in the polarization of T-cells and in the function of regulatory T (Treg)-cells. We set out to study whether an impaired induction of regulatory mechanisms during the generation of T-cell responses upon stimulation is associated with T1D.

Methods

Naive T-cells were isolated from 18 children with recent T1D (0–14days from diagnosis; mean age 9.3 years), 11 children who had had T1D for at least 1 year (mean age 10.6) and 14 non-diabetic children (mean age 8.1). CD45RA+ T-cells were stimulated with PHA for 72 h in type 1 cytokine [interleukin (IL)-12 and anti-IL-4] or type 2 cytokine (IL-4 and anti-IL-12) environment. T-cell polarization and regulation related markers were analysed by quantitative reverse transcription polymerase chain reaction (QRT-PCR) (Th1 promoting T-bet, Th2 promoting GATA-3 and regulation related FOXP3, ICOS and NFATc2).

Results

Children with recently diagnosed T1D showed decreased induction of FOXP3, ICOS and NFATc2 in T-cells activated in type 1 cytokine milieu (p = 0.007, p = 0.001, and p = 0.02), whereas no differences between the diabetic and healthy children were seen in the up-regulation of activation markers, T-bet and GATA-3.

Conclusions

The poor induction of factors that mediate down-modulation of T-cell responses upon stimulation in type 1 cytokine environment may contribute to the development of autoreactive type 1 responses in T1D.

Place, publisher, year, edition, pages
2008. Vol. 24, no 8, 635-641 p.
Keyword [en]
type 1 diabetes, T helper cells, regulatory T-cell, naive T-cell, quantitative RT-PCR
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-16217DOI: 10.1002/dmrr.904OAI: oai:DiVA.org:liu-16217DiVA: diva2:133460
Available from: 2009-01-12 Created: 2009-01-09 Last updated: 2017-12-14Bibliographically approved

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Skarsvik, SusanneVaarala, Outi

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