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Control of Neural Stem Cell Adhesion and Density by an Electronic Polymer Surface Switch
Karolinska Institute.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
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2008 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 24, no 24, 14133-14138 p.Article in journal (Refereed) Published
Abstract [en]

Adhesion is an essential parameter for stem cells. It regulates the overall cell density along the carrying surface, which further dictates the differentiation scheme of stem cells toward a more matured and specified population as well as tissue. Electronic control of the seeding density of neural stem cells (c17.2) is here reported. Thin electrode films of poly(3,4-ethylenedioxythiophene) (PEDOT):Tosylate were manufactured along the floor of cell growth dishes. As the oxidation state of the conjugated polymer electrodes was controlled, the seeding density could be varied by a factor of 2. Along the oxidized PEDOT:Tosylate-electrodes, a relatively lower density of, and less tightly bonded, human serum albumin (HSA) was observed as compared to reduced electrodes. We found that this favors adhesion of the specific stem cells studied. Surface analysis experiments, such as photoelectron spectroscopy, and water contact angle measurements, were carried out to investigate the mechanisms responsible for the electronic control of the seeding density of the c17.2 neural stem cells. Further, our findings may provide an opening for electronic control of stem cell differentiation.

Place, publisher, year, edition, pages
2008. Vol. 24, no 24, 14133-14138 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-16247DOI: 10.1021/la8028337OAI: oai:DiVA.org:liu-16247DiVA: diva2:133516
Available from: 2009-01-12 Created: 2009-01-09 Last updated: 2017-12-14
In thesis
1. Conjugated Polymer Surface Switches for Active Control
Open this publication in new window or tab >>Conjugated Polymer Surface Switches for Active Control
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polymers have been found useful in a wide range of applications such as sensors, electrochemical transistors, solar cells, and printed electronics due to their mechanical, optical and electronic properties. An amazing research field has grown during the last three decades since the discovery of conducting polymers in 1976. Since the materials can be made from solutions, different processing methods such as spin coating and vapor phase polymerization can be used to coat a huge variety of substrates. The choice of method depends mainly on monomer solubility and kind of substrate to be coated. During the synthesis the polymers can be chemically modified to tailor their functionalities. Due to this variability in materials and the processability, electronics can be achieved on unconventional substrates such as flexible plastic foils and cell culturing dishes. As a contrast to inorganic, usually metallic materials, conducting polymers are built up from organic compounds in a molecular structure with soft mechanical properties that have shown to be a benefit in combination with biology, ranging from interactions with cells to interactions with advanced biological species such as tissues. This combination of research fields and the possible applications are merged within the field of organic bioelectronics.

The primary purpose of this thesis is to give a background to organic electronics in general and how electrochemical devices can be processed and developed for biological applications in particular. An organic electronic surface switch is introduced to control cell adhesion and proliferation as well as an electrochemical transistor to spatially tune the cell adhesion along an electrochemical gradient. To mimic a more natural cell environment a three dimensional fiber substrate was used to design an electronically active matrix to promote nerve cell adhesion and communication. By combining standard microfabrication techniques and conjugated polymers desired patterns of electroactive polymer were created to enable active regulation of cell populations and their extracellular environment at high spatial resolution. Finally, a brief look into future challenges will also be presented.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2011. 52 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1398
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-71361 (URN)978-91-7393-063-5 (ISBN)
Public defence
2011-10-21, K3, Kåkenhus, Campus Norrköping, Linköpings universitet, Norrköping, 13:15 (English)
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Available from: 2011-10-13 Created: 2011-10-13 Last updated: 2017-02-03Bibliographically approved

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Saindon, EmilienBolin, MariaKanciurzewska, AnnaFahlman, MatsJager, EdwinTengvall, PenttiBerggren , Magnus

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Saindon, EmilienBolin, MariaKanciurzewska, AnnaFahlman, MatsJager, EdwinTengvall, PenttiBerggren , Magnus
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