liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Feeding of mice with Arabidopsis thaliana expressing the HIV-1 subtype C p24 antigen gives rise to systemic immune responses
Örebro University.
Örebro University.
Örebro University.
Örebro University.
Show others and affiliations
2008 (English)In: APMIS: Acta pathologica, microbiologica et immunologica Scandinavica. Supplementum, ISSN 0903-465X, Vol. 116, no 11, 985-994 p.Article in journal (Refereed) Published
Abstract [en]

Development of transgenic edible plants, to be used as production, storage and delivery systems for recombinant vaccine antigens, is a promising strategy to obtain cost effective vaccines against infectious diseases, not least for use in developing countries. Therefore, we used Agrobacterium tumefaciens-mediated gene transfer to introduce the p24 gag gene encoding the nucleocapsid protein from HIV-1 subtype C into the Arabidopsis thaliana plant genome. Eighteen plant lines were confirmed positive for the p24 gene by PCR; four of these lines showed an apparent homozygous phenotype when grown on selective medium and these lines also showed transcription of the p24 gene into its corresponding mRNA. The mRNA in all four cases generated the p24 protein in plants, as verified by Western blot analysis. The plants were shown to contain between 0.2 mu g and 0.5 mu g p24 protein per g of fresh tissue. Analysis of the localisation of the p24 protein showed that stem tissue contained the largest amount of protein, more than twice as much as leaf tissue, whereas no p24 protein was detected in roots. By using Southern blotting, we found that 4, 2-3, 2 and 1 T-DNA insertion events took place in the four lines 1, 2, 7, and 10, respectively. The genetic insertions of line 1 were stable from the T-2 to the T-5 generation and gave rise to the p24 protein in all cases, as verified by Western blotting. In mice fed with fresh transgenic A. thaliana (line 10), anti-gag IgG was obtained in serum after a booster injection with recombinant p37Gag. No immune response was observed after equal booster injection of untreated mice or mice fed with A. thaliana WT plants.

Place, publisher, year, edition, pages
2008. Vol. 116, no 11, 985-994 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-16359DOI: 10.1111/j.1600-0463.2008.00900.xOAI: oai:DiVA.org:liu-16359DiVA: diva2:133976
Note
The definitive version is available at www.blackwell-synergy.com: Ingrid Lindh, Irina Kalbina, Sara Thulin, Nikolai Scherbak, Helena Savenstrand, Andreas Brave, Jorma Hinkula, Ake Strid and Soren Andersson, Feeding of mice with Arabidopsis thaliana expressing the HIV-1 subtype C p24 antigen gives rise to systemic immune responses, 2008, APMIS, (116), 11, 985-994. http://dx.doi.org/10.1111/j.1600-0463.2008.00900.x Copyright: Blackwell Publishing Ltd http://www.blackwellpublishing.com/ Available from: 2009-01-28 Created: 2009-01-16 Last updated: 2009-05-06Bibliographically approved

Open Access in DiVA

fulltext(248 kB)449 downloads
File information
File name FULLTEXT01.pdfFile size 248 kBChecksum SHA-512
c0a45020de1106ca6f66de07805b1aeb6dc4d35c2836ba92ba305d8217f5cd541bcda64888899059f49b4c6b1a17d348453e6f863e0224aa67fbd442245f08db
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Hinkula, Jorma

Search in DiVA

By author/editor
Hinkula, Jorma
By organisation
Department of Clinical and Experimental MedicineFaculty of Health Sciences
In the same journal
APMIS: Acta pathologica, microbiologica et immunologica Scandinavica. Supplementum
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 449 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 100 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf