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Application of Bioactive Thermal Proteome Profiling to Decipher the Mechanism of Action of the Lipid Lowering 13(2)-Hydroxy-pheophytin Isolated from a Marine Cyanobacteria
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Interdisciplinary Ctr Marine and Environm Res, Portugal.
Interdisciplinary Ctr Marine and Environm Res, Portugal.
Univ Basque Country, Spain.
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2019 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 17, no 6Article in journal (Refereed) Published
Abstract [en]

The acceleration of the process of understanding the pharmacological application of new marine bioactive compounds requires identifying the compound protein targets leading the molecular mechanisms in a living cell. The thermal proteome profiling (TPP) methodology does not fulfill the requirements for its application to any bioactive compound lacking chemical and functional characterization. Here, we present a modified method that we called bTPP for bioactive thermal proteome profiling that guarantees target specificity from a soluble subproteome. We showed that the precipitation of the microsomal fraction before the thermal shift assay is crucial to accurately calculate the melting points of the protein targets. As a probe of concept, the protein targets of 13(2)-hydroxy-pheophytin, a compound previously isolated from a marine cyanobacteria for its lipid reducing activity, were analyzed on the hepatic cell line HepG2. Our improved method identified 9 protein targets out of 2500 proteins, including 3 targets (isocitrate dehydrogenase, aldehyde dehydrogenase, phosphoserine aminotransferase) that could be related to obesity and diabetes, as they are involved in the regulation of insulin sensitivity and energy metabolism. This study demonstrated that the bTPP method can accelerate the field of biodiscovery, revealing protein targets involved in mechanisms of action (MOA) connected with future applications of bioactive compounds.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 17, no 6
Keywords [en]
thermal proteome profiling; mechanisms of action; bioactive compound; label-free quantitative proteomics; marine biodiscovery
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-159281DOI: 10.3390/md17060371ISI: 000475305700060PubMedID: 31234367OAI: oai:DiVA.org:liu-159281DiVA, id: diva2:1341130
Note

Funding Agencies|ERA-NET Marine Biotechnology project CYANOBESITY; FORMAS, Sweden [2016-02004]; FCT Foundation of Science and Technology, Portugal [ERA-MBT/0001/2015]; IKERBASQUE; Basque Government [IT-971-16]; FCT [SFRH/BPD/112287/2015, SFRH/BD/116009/2016, UID/Multi/04423/2019]

Available from: 2019-08-07 Created: 2019-08-07 Last updated: 2019-08-07

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Carrasco Del Amor, Ana MariaCristobal, Susana
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